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BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation.
Campagne, Antoine; Lee, Ming-Kang; Zielinski, Dina; Michaud, Audrey; Le Corre, Stéphanie; Dingli, Florent; Chen, Hong; Shahidian, Lara Z; Vassilev, Ivaylo; Servant, Nicolas; Loew, Damarys; Pasmant, Eric; Postel-Vinay, Sophie; Wassef, Michel; Margueron, Raphaël.
Afiliación
  • Campagne A; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Lee MK; INSERM U934/CNRS UMR3215, 75005, Paris, France.
  • Zielinski D; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Michaud A; INSERM U934/CNRS UMR3215, 75005, Paris, France.
  • Le Corre S; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Dingli F; INSERM U934/CNRS UMR3215, 75005, Paris, France.
  • Chen H; INSERM U900, Mines ParisTech, 75005, Paris, France.
  • Shahidian LZ; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Vassilev I; INSERM U934/CNRS UMR3215, 75005, Paris, France.
  • Servant N; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Loew D; INSERM U934/CNRS UMR3215, 75005, Paris, France.
  • Pasmant E; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Postel-Vinay S; Institut Curie, Paris Sciences et Lettres Research University, Sorbonne University, 75005, Paris, France.
  • Wassef M; INSERM U934/CNRS UMR3215, 75005, Paris, France.
  • Margueron R; Institute of Functional Epigenetics, Helmholtz Zentrum München, Neuherberg, 85764, Germany.
Nat Commun ; 10(1): 348, 2019 01 21.
Article en En | MEDLINE | ID: mdl-30664650
In Drosophila, a complex consisting of Calypso and ASX catalyzes H2A deubiquitination and has been reported to act as part of the Polycomb machinery in transcriptional silencing. The mammalian homologs of these proteins (BAP1 and ASXL1/2/3, respectively), are frequently mutated in various cancer types, yet their precise functions remain unclear. Using an integrative approach based on isogenic cell lines generated with CRISPR/Cas9, we uncover an unanticipated role for BAP1 in gene activation. This function requires the assembly of an enzymatically active BAP1-associated core complex (BAP1.com) containing one of the redundant ASXL proteins. We investigate the mechanism underlying BAP1.com-mediated transcriptional regulation and show that it does not participate in Polycomb-mediated silencing. Instead, our results establish that the function of BAP1.com is to safeguard transcriptionally active genes against silencing by the Polycomb Repressive Complex 1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Histonas / Procesamiento Proteico-Postraduccional / Proteínas de Ciclo Celular / Proteínas Supresoras de Tumor / Ubiquitina Tiolesterasa Límite: Animals / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos B / Histonas / Procesamiento Proteico-Postraduccional / Proteínas de Ciclo Celular / Proteínas Supresoras de Tumor / Ubiquitina Tiolesterasa Límite: Animals / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido