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Alpha-glucosidase inhibitor 1-Deoxynojirimycin promotes beige remodeling of 3T3-L1 preadipocytes via activating AMPK.
Li, An-Na; Chen, Jun-Jun; Li, Qing-Qing; Zeng, Guang-Yao; Chen, Qian-Yun; Chen, Jun-Ling; Liao, Zhi-Mei; Jin, Ping; Wang, Kuan-Song; Yang, Zhi-Chun.
Afiliación
  • Li AN; Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
  • Chen JJ; Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
  • Li QQ; Department of Pharmacology, Changsha Medical University, Changsha, 410219, China.
  • Zeng GY; Department of Pharmacochemistry, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
  • Chen QY; Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
  • Chen JL; Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
  • Liao ZM; Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China.
  • Jin P; Department of Endocrinology, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
  • Wang KS; Department of Pathology, The Xiangya Hospital, Central South University, Changsha, 410008, China.
  • Yang ZC; Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China; Hunan Provincial Key Laboratory of Cardiovascular Research, Changsha, 410078, China. Electronic address: yzhichun@csu.edu.cn.
Biochem Biophys Res Commun ; 509(4): 1001-1007, 2019 02 19.
Article en En | MEDLINE | ID: mdl-30654939
Obesity is a serious health challenge in the world, and searching effective drugs to cure obesity is of great importance. 1-Deoxynojirimycin (DNJ) is extracted from mulberry leaves and acts as an α-glucosidase inhibitor to lower blood glucose. Recent studies demonstrated that it also has anti-obesity effect, but the mechanisms remain unknown. In our present study, we mainly examined the effects of DNJ on beige remodeling of 3T3-L1 preadipocytes. We observed that DNJ didn't affect the mRNA levels of fatty acid binding protein 4 (aP2), peroxisome proliferator-activated receptor γ (PPARγ), preadipocyte factor-1 (Pref-1) as well as the mitochondrial uncoupling protein 1 (UCP1), PR domain containing protein 16 (PRDM16), transmembrane protein 26 (TMEM26) in undifferentiated preadipocytes. But after inducing 3T3-L1 preadipocytes to differentiation with white or beige adipogenic medium, DNJ significantly reduced aP2, PPARγ and Pref-1 expressions, while up-regulated the expressions of UCP1, PRDM16 and TMEM26, accompanying with decreased lipid deposition. The ratio of p-AMPK/AMPK was up-regulated by DNJ (10 µM) treatment for 10 days, and the effects of DNJ on p-AMPK/AMPK, UCP1 and PRDM16 could be blocked by AMPK inhibitor Compound C. These results demonstrated that hypoglycemic agent DNJ could suppress the adipogenesis during the differentiation of white preadipocytes, and promote the switch of white preadipocytes to beige adipocytes via activating AMPK, which provided new mechanisms for explaining the benefits of DNJ on obesity-related disorders.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: 1-Desoxinojirimicina / Adipocitos / Proteínas Quinasas Activadas por AMP / Inhibidores de Glicósido Hidrolasas / Adipocitos Beige / Obesidad Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: 1-Desoxinojirimicina / Adipocitos / Proteínas Quinasas Activadas por AMP / Inhibidores de Glicósido Hidrolasas / Adipocitos Beige / Obesidad Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos