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Biomechanical Stability and Osteogenesis in a Tibial Bone Defect Treated by Autologous Ovine Cord Blood Cells-A Pilot Study.
Herten, Monika; Zilkens, Christoph; Thorey, Fritz; Tassemeier, Tjark; Lensing-Höhn, Sabine; Fischer, Johannes C; Sager, Martin; Krauspe, Rüdiger; Jäger, Marcus.
Afiliación
  • Herten M; Department of Orthopedics and Trauma Surgery, University of Duisburg-Essen, 45147 Essen, Germany. Monika.Herten@uk-essen.de.
  • Zilkens C; Orthopedic Department, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany. Christoph.zilkens@med.uni-duesseldorf.de.
  • Thorey F; Center for Hip, Knee and Foot Surgery, Sports Traumatology Department, ATOS Hospital, 69115 Heidelberg, Germany. Fritz.Thorey@atos.de.
  • Tassemeier T; Department of Orthopedics and Trauma Surgery, University of Duisburg-Essen, 45147 Essen, Germany. Tjark.Tassemeier@uk-essen.de.
  • Lensing-Höhn S; Orthopedic Department, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany. Sabine.Lensing-Hoehn@med.uni-duesseldorf.de.
  • Fischer JC; Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany. Johannes.Fischer@med.uni-duesseldorf.de.
  • Sager M; Animal Research Institute, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany. Martin.Sager@med.uni-duesseldorf.de.
  • Krauspe R; Orthopedic Department, Heinrich-Heine-University Düsseldorf, 40225 Düsseldorf, Germany. Ruediger.Krauspe@med.uni-duesseldorf.de.
  • Jäger M; Department of Orthopedics and Trauma Surgery, University of Duisburg-Essen, 45147 Essen, Germany. Marcus.Jaeger@uk-essen.de.
Molecules ; 24(2)2019 Jan 15.
Article en En | MEDLINE | ID: mdl-30650584
The aim of this study was to elucidate the impact of autologous umbilical cord blood cells (USSC) on bone regeneration and biomechanical stability in an ovine tibial bone defect. Ovine USSC were harvested and characterized. After 12 months, full-size 2.0 cm mid-diaphyseal bone defects were created and stabilized by an external fixateur containing a rigidity measuring device. Defects were filled with (i) autologous USSC on hydroxyapatite (HA) scaffold (test group), (ii) HA scaffold without cells (HA group), or (iii) left empty (control group). Biomechanical measures, standardized X-rays, and systemic response controls were performed regularly. After six months, bone regeneration was evaluated histomorphometrically and labeled USSC were tracked. In all groups, the torsion distance decreased over time, and radiographies showed comparable bone regeneration. The area of newly formed bone was 82.5 ± 5.5% in the control compared to 59.2 ± 13.0% in the test and 48.6 ± 2.9% in the HA group. Labeled cells could be detected in lymph nodes, liver and pancreas without any signs of tumor formation. Although biomechanical stability was reached earliest in the test group with autologous USSC on HA scaffold, the density of newly formed bone was superior in the control group without any bovine HA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Tibia / Regeneración Ósea / Sangre Fetal Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Tibia / Regeneración Ósea / Sangre Fetal Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Suiza