Characterization of Pleurotus djamor neohaplonts recovered by production of protoplasts and chemical dedikaryotization.
3 Biotech
; 9(1): 24, 2019 Jan.
Article
en En
| MEDLINE
| ID: mdl-30622862
Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
3 Biotech
Año:
2019
Tipo del documento:
Article
Pais de publicación:
Alemania