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Coupling Self-Assembly Mechanisms to Fabricate Molecularly and Electrically Responsive Films.
Li, Jinyang; Maniar, Drishti; Qu, Xue; Liu, Huan; Tsao, Chen-Yu; Kim, Eunkyoung; Bentley, William E; Liu, Changsheng; Payne, Gregory F.
Afiliación
  • Li J; Institute for Bioscience and Biotechnology Research , University of Maryland , College Park , Maryland 20742 , United States.
  • Maniar D; Fischell Department of Bioengineering , University of Maryland , College Park , Maryland 20742 , United States.
  • Qu X; Institute for Bioscience and Biotechnology Research , University of Maryland , College Park , Maryland 20742 , United States.
  • Liu H; Fischell Department of Bioengineering , University of Maryland , College Park , Maryland 20742 , United States.
  • Tsao CY; Key Laboratory for Ultrafine Materials of Ministry of Education, The State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , 200237 , China.
  • Kim E; Key Laboratory for Ultrafine Materials of Ministry of Education, The State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , 200237 , China.
  • Bentley WE; Institute for Bioscience and Biotechnology Research , University of Maryland , College Park , Maryland 20742 , United States.
  • Liu C; Fischell Department of Bioengineering , University of Maryland , College Park , Maryland 20742 , United States.
  • Payne GF; Institute for Bioscience and Biotechnology Research , University of Maryland , College Park , Maryland 20742 , United States.
Biomacromolecules ; 20(2): 969-978, 2019 02 11.
Article en En | MEDLINE | ID: mdl-30616349
Biomacromolecules often possess information to self-assemble through low energy competing interactions which can make self-assembly responsive to environmental cues and can also confer dynamic properties. Here, we coupled self-assembling systems to create biofunctional multilayer films that can be cued to disassemble through either molecular or electrical signals. To create functional multilayers, we: (i) electrodeposited the pH-responsive self-assembling aminopolysaccharide chitosan, (ii) allowed the lectin Concanavalin A (ConA) to bind to the chitosan-coated electrode (presumably through electrostatic interactions), (iii) performed layer-by-layer self-assembly by sequential contacting with glycogen and ConA, and (iv) conferred biological (i.e., enzymatic) function by assembling glycoprotein (i.e., enzymes) to the ConA-terminated multilayer. Because the ConA tetramer dissociates at low pH, this multilayer can be triggered to disassemble by acidification. We demonstrate two approaches to induce acidification: (i) glucose oxidase can induce multilayer disassembly in response to molecular cues, and (ii) anodic reactions can induce multilayer disassembly in response to electrical cues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sustancias Macromoleculares Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sustancias Macromoleculares Idioma: En Revista: Biomacromolecules Asunto de la revista: BIOLOGIA MOLECULAR Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos