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Dengue virus requires apoptosis linked gene-2-interacting protein X (ALIX) for viral propagation.
Thepparit, Chutima; Khongwichit, Sarawut; Ketsuwan, Kunjimas; Libsittikul, Sirikwan; Auewarakul, Prasert; Smith, Duncan R.
Afiliación
  • Thepparit C; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand.
  • Khongwichit S; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand.
  • Ketsuwan K; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand.
  • Libsittikul S; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand.
  • Auewarakul P; Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Rd, Bangkoknoi, Bangkok 10700, Thailand.
  • Smith DR; Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakhon Pathom, Bangkok, 73170, Thailand. Electronic address: duncan.smi@mahidol.ac.th.
Virus Res ; 261: 65-71, 2019 02.
Article en En | MEDLINE | ID: mdl-30599162
The endosomal sorting complexes required for transport (ESCRT) pathway accessory protein apoptosis linked gene-2-interacting protein X (ALIX) has been shown to be upregulated during dengue virus (DENV) replication. Yeast-two-hybrid screens have additionally shown that ALIX interacts with DENV NS3 protein, but evaluation of the interaction through a replicon assay failed to show a functional significance to the interaction. In this study the interaction between DENV NS3 and ALIX was investigated by co-immunoprecipitation, and functional significance assessed by investigation of DENV production in ALIX expression regulated cells. The results showed that ALIX both interacted and co-localized with DENV NS3 protein and that upregulation of ALIX resulted in a significantly increased viral titer, while either siRNA or CRISPR-Cas9 mediated down regulation of ALIX significantly reduced viral production, without affecting relative DENV genome levels. These results are consistent with ALIX playing a significant role in the DENV replication cycle either during late infection or at viral egress.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Proteínas de Unión al Calcio / Proteínas no Estructurales Virales / Proteínas de Ciclo Celular / Virus del Dengue / Interacciones Huésped-Patógeno / Complejos de Clasificación Endosomal Requeridos para el Transporte Límite: Humans Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Proteínas de Unión al Calcio / Proteínas no Estructurales Virales / Proteínas de Ciclo Celular / Virus del Dengue / Interacciones Huésped-Patógeno / Complejos de Clasificación Endosomal Requeridos para el Transporte Límite: Humans Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Países Bajos