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Characterization of meningococcal carriage isolates from Greece by whole genome sequencing: Implications for 4CMenB vaccine implementation.
Kesanopoulos, Konstantinos; Bratcher, Holly B; Hong, Eva; Xirogianni, Athanasia; Papandreou, Anastasia; Taha, Muhamed-Kheir; Maiden, Martin C J; Tzanakaki, Georgina.
Afiliación
  • Kesanopoulos K; National Meningitis Reference Laboratory (NMRL), Dept of Public Health, National School of Public Health, Athens, Greece.
  • Bratcher HB; Department of Zoology, Peter Medawar Building, University of Oxford, Oxford, United Kingdom.
  • Hong E; Institute Pasteur, Invasive Bacterial Infections Unit, Paris, France.
  • Xirogianni A; National Meningitis Reference Laboratory (NMRL), Dept of Public Health, National School of Public Health, Athens, Greece.
  • Papandreou A; National Meningitis Reference Laboratory (NMRL), Dept of Public Health, National School of Public Health, Athens, Greece.
  • Taha MK; Institute Pasteur, Invasive Bacterial Infections Unit, Paris, France.
  • Maiden MCJ; Department of Zoology, Peter Medawar Building, University of Oxford, Oxford, United Kingdom.
  • Tzanakaki G; National Meningitis Reference Laboratory (NMRL), Dept of Public Health, National School of Public Health, Athens, Greece.
PLoS One ; 13(12): e0209919, 2018.
Article en En | MEDLINE | ID: mdl-30592763
Herd protection, resulting from the interruption of transmission and asymptomatic carriage, is an important element of the effectiveness of vaccines against the meningococcus. Whilst this has been well established for conjugate polysaccharide vaccines directed against the meningococcal capsule, two uncertainties surround the potential herd protection provided by the novel protein-based vaccines that are used in place of serogroup B (MenB) polysaccharide vaccines (i) the strain coverage of such vaccines against carried meningococci, which are highly diverse; and (ii) the generation of a protective immune response in the mucosa. These considerations are essential for realistic estimates of cost-effectiveness of new MenB vaccines. Here the first of these questions is addressed by the whole genome sequence (WGS) analysis of meningococci isolated from healthy military recruits and university students in Greece. The study included a total of 71 MenB isolates obtained from 1420 oropharyngeal single swab samples collected from military recruits and university students on voluntary basis, aged 18-26 years. In addition to WGS analysis to identify genetic lineage and vaccine antigen genes, including the Bexsero Antigen Sequence Type (BAST), the isolates were examined with the serological Meningococcal antigen Typing System (MATS) assay. Comparison of these data demonstrated that the carried meningococcal population was highly diverse with 38% of the carriage isolates showed expression of antigens matching those included in the 4CMenB vaccine. Our data may suggest a limited potential herd immunity to be expected and be driven by an impact on a subset of carriage isolates.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Genoma Bacteriano / Vacunas Meningococicas / Neisseria meningitidis Serogrupo B / Estudio de Asociación del Genoma Completo / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Female / Humans / Male País/Región como asunto: Europa Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Variación Genética / Genoma Bacteriano / Vacunas Meningococicas / Neisseria meningitidis Serogrupo B / Estudio de Asociación del Genoma Completo / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Female / Humans / Male País/Región como asunto: Europa Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Estados Unidos