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Drosophila Sister-of-Sex-lethal reinforces a male-specific gene expression pattern by controlling Sex-lethal alternative splicing.
Moschall, Rebecca; Rass, Mathias; Rossbach, Oliver; Lehmann, Gerhard; Kullmann, Lars; Eichner, Norbert; Strauss, Daniela; Meister, Gunter; Schneuwly, Stephan; Krahn, Michael P; Medenbach, Jan.
Afiliación
  • Moschall R; Institute of Biochemistry I, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Rass M; Institute of Zoology, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Rossbach O; Institute of Biochemistry, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen, Germany.
  • Lehmann G; Institute of Biochemistry I, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Kullmann L; Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Eichner N; Institute of Biochemistry I, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Strauss D; Institute of Biochemistry I, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Meister G; Institute of Biochemistry I, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Schneuwly S; Institute of Zoology, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Krahn MP; Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitaetsstrasse 31, D-93053 Regensburg, Germany.
  • Medenbach J; Medical Clinic D, University of Muenster, Domagkstrasse 3, D-48149 Muenster, Germany.
Nucleic Acids Res ; 47(5): 2276-2288, 2019 03 18.
Article en En | MEDLINE | ID: mdl-30590805
In Drosophila, female development is governed by a single RNA-binding protein, Sex-lethal (Sxl), that controls the expression of key factors involved in dosage compensation, germline homeostasis and the establishment of female morphology and behaviour. Sxl expression in female flies is maintained by an auto-regulatory, positive feedback loop with Sxl controlling splicing of its own mRNA. Until now, it remained unclear how males prevent accidental triggering of the Sxl expression cascade and protect themselves against runaway protein production. Here, we identify the protein Sister-of-Sex-lethal (Ssx) as an inhibitor of Sxl auto-regulatory splicing. Sxl and Ssx have a comparable RNA-binding specificity and compete for binding to RNA regulatory elements present in the Sxl transcript. In cultured Drosophila cells, Sxl-induced changes to alternative splicing can be reverted by the expression of Ssx. Moreover, in adult male flies ablation of the ssx gene results in a low level of productive Sxl mRNA splicing and Sxl protein production in isolated, clonal cell populations. In sum, this demonstrates that Ssx safeguards male animals against Sxl protein production to reinforce a stable, male-specific gene expression pattern.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caracteres Sexuales / Proteínas de Unión al ARN / Empalme Alternativo / Regulación del Desarrollo de la Expresión Génica / Proteínas de Drosophila / Drosophila melanogaster Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Nucleic Acids Res Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Caracteres Sexuales / Proteínas de Unión al ARN / Empalme Alternativo / Regulación del Desarrollo de la Expresión Génica / Proteínas de Drosophila / Drosophila melanogaster Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Nucleic Acids Res Año: 2019 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido