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The prevalence and clinical significance of microcolonies when tested according to contemporary interpretive breakpoints for fluconazole against Candida species using E-test.
Hauzer, Michael; Cohen, Matan J; Polacheck, Itzhack; Moses, Allon; Korem, Maya.
Afiliación
  • Hauzer M; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
  • Cohen MJ; Clalit Health Services, Jerusalem, Israel.
  • Polacheck I; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
  • Moses A; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
  • Korem M; Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.
Med Mycol ; 57(6): 718-723, 2019 Aug 01.
Article en En | MEDLINE | ID: mdl-30576514
Changes in the interpretive-breakpoints for antifungals against various Candida species have raised the need to examine the significance of the phenomenon of the growth of microcolonies in agar diffusion inhibition zones, which has generally been considered negligible. The objective was to determine the incidence of cases in which microcolonies demonstrate fluconazole resistance according to current interpretive-breakpoints and whether their growth is associated with therapeutic failure. The fluconazole minimum inhibitory concentrations (MICs) of 100 blood culture isolates of Candida were performed by E-test on Roswell Park Memorial Institute (RPMI) agar and examined for the appearance of microcolonies. Fluconazole MICs of microcolonies were then determined over three generations. The significance of the phenomenon of microcolonies was determined according to clinical data retrieved from electronic files. Microcolonies were a common phenomenon among Candida isolates following incubation on RPMI agar, with a higher frequency among C. albicans isolates as compared to non-albicans Candida across generations (57-93% vs 31-93%, respectively) and a similar fluconazole susceptibility rate over three generations. The rate of microcolonies was similar in both patients with successful and unsuccessful outcome (41% vs 42%, respectively). Microcolonies are a common phenomenon. No increase in MIC was demonstrated throughout three generations of microcolony inoculation on RPMI, and no difference in clinical outcome was observed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Fluconazol / Antifúngicos Tipo de estudio: Incidence_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Med Mycol Asunto de la revista: MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida / Fluconazol / Antifúngicos Tipo de estudio: Incidence_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Med Mycol Asunto de la revista: MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Reino Unido