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Evaluation of a Novel Stool Antigen Rapid Test Kit for Detection of Helicobacter pylori Infection.
Kakiuchi, Toshihiko; Okuda, Masumi; Hashiguchi, Kazutoshi; Imamura, Ichiro; Nakayama, Aiko; Matsuo, Muneo.
Afiliación
  • Kakiuchi T; Department of Pediatrics, Faculty of Medicine, Saga University, Saga, Japan kakiucht@cc.saga-u.ac.jp.
  • Okuda M; Department of Pediatrics, Aichi Medical University School of Medicine, Nagakute, Japan.
  • Hashiguchi K; Department of General Medicine and Community Health Science, Hyogo College of Medicine, Sasayama, Japan.
  • Imamura I; Division of Gastroenterology, Department of Internal Medicine, Imamura Hospital, Tosu, Japan.
  • Nakayama A; Division of Gastroenterology, Department of Internal Medicine, Imamura Hospital, Tosu, Japan.
  • Matsuo M; Department of Pediatrics, Faculty of Medicine, Saga University, Saga, Japan.
J Clin Microbiol ; 57(3)2019 03.
Article en En | MEDLINE | ID: mdl-30567746
The Quick Chaser H. pylori (QCP), which is a novel antigen detection kit for Helicobacter pylori, was developed recently. The previous examination kits targeted the catalase of H. pylori; however, this new test kit, to the best of our knowledge, is the first kit to target the flagellar protein of H. pylori The present study aimed to evaluate the efficacy of QCP compared with that of the already commercially available rapid test kit Testmate Rapid Pylori Antigen (TRP). TRP and QCP were utilized in 71 participants. The positive and negative concordance ratios of QCP to TRP were 100% (57/57) and 92.9% (13/14), respectively. Sensitivity based on the rapid urease test and culture test was 92.3%. Results of the dilution sensitivity test showed that QCP was eight times more sensitive to an H. pylori standard strain and the clarithromycin (CAM)-resistant clinical isolate and four times more sensitive to a CAM-susceptible clinical isolate than TRP. For the cross-reactivity test, 27 strains that exist in human feces, such as the Helicobacter genus, Bifidobacterium genus, Lactobacillus genus, and other resident bacteria, were selected. The results obtained using QCP were all negative, and no cross-reaction was observed. In conclusion, compared with TRP, QCP can detect H. pylori using clinical specimens with high sensitivity, regardless of CAM susceptibility and tolerance. No cross-reactivity was observed with other intestinal bacteria in humans. The kit is considered extremely useful in clinical settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter / Heces / Antígenos Bacterianos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Clin Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Helicobacter pylori / Infecciones por Helicobacter / Heces / Antígenos Bacterianos Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Clin Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos