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The Motion of An Inv Nodal Cilium: a Realistic Model Revealing Dynein-Driven Ciliary Motion with Microtubule Mislocalization.
Yu, Yanan; Shinohara, Kyosuke; Xu, Huanming; Li, Zhenfeng; Nishida, Tomoki; Hamada, Hiroshi; Xu, Yuanqing; Zhou, Jingqi; Shao, Daisy; Li, Xiangchen; Chen, Duanduan.
Afiliación
  • Yu Y; School of Life Science, Beijing Institute of Technology, Beijing, China.
  • Shinohara K; Department of Biotechnology and Life Science, Tokyo University of Agriculture & Technology, Tokyo, Japan.
  • Xu H; School of Life Science, Beijing Institute of Technology, Beijing, China.
  • Li Z; School of Life Science, Beijing Institute of Technology, Beijing, China.
  • Nishida T; Japan Textile Products Quality and Technology Centre, Kobe, Japan.
  • Hamada H; Laboratory for Organismal Patterning, RIKEN Centre for Developmental Biology, Kobe, Japan.
  • Xu Y; School of Life Science, Beijing Institute of Technology, Beijing, China.
  • Zhou J; Chongqing Nankai Secondary School, Chongqing, China.
  • Shao D; Hyde Park Baptist High School, Austin, Texas, USA.
  • Li X; Beijing the Eighth High School, Beijing, China.
  • Chen D; School of Life Science, Beijing Institute of Technology, Beijing, Chinaduanduan@bit.edu.cn.
Cell Physiol Biochem ; 51(6): 2843-2857, 2018.
Article en En | MEDLINE | ID: mdl-30562762
BACKGROUND/AIMS: Nodal cilia that rotate in the ventral node play an important role in establishing left-right asymmetry during embryogenesis; however, inv mutant cilia present abnormal movement and induce laterality defects. The mechanism of their motility, which is regulated by dynein activation and microtubule arrangement, has not been fully understood. This study analyzed the dynein-triggered ciliary motion in the abnormal ultrastructure of the inv mutant, aiming to quantitatively evaluate the influence of microtubule mislocalization on the movement of the cilium. METHODS: We established a realistic 3-D model of an inv mutant cilium with an ultrastructure based on tomographic datasets generated by ultra-high voltage electron microscopy. The time-variant activation of the axonemal dynein force was simulated by pairs of point loads and embedded at dynein-mounted positions between adjacent microtubule doublets in this mathematical model. Utilizing the finite element method and deformable grid, the motility of the mutant cilium that is induced by various dynein activation hypotheses was investigated and compared to experimental observation. RESULTS: The results indicate that for the inv mutant, simulations of the ciliary movement with the engagement of dyneins based on the distance-controlled pattern in the partially activation scenario are broadly consistent with the observation; the shortening of the microtubules induces smaller movement amplitudes, while the angles of the mislocalized microtubules affect the pattern of the ciliary movement, and during the ciliary movement, the microtubules swing and twist in the mutant ciliary body. CONCLUSION: More generally, this study implies that dynein engagement is sensitive to subtle geometric changes in the axoneme, and thus, this geometry greatly influences the integrity of a well-formed ciliary rotation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cilios / Dineínas / Microtúbulos Límite: Animals Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cilios / Dineínas / Microtúbulos Límite: Animals Idioma: En Revista: Cell Physiol Biochem Asunto de la revista: BIOQUIMICA / FARMACOLOGIA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania