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Cell-Type-Specific Proteomics: A Neuroscience Perspective.
Wilson, Rashaun S; Nairn, Angus C.
Afiliación
  • Wilson RS; Yale/NIDA Neuroproteomics Center, 300 George St., New Haven, CT 06511, USA. rashaun.wilson@yale.edu.
  • Nairn AC; Yale/NIDA Neuroproteomics Center, 300 George St., New Haven, CT 06511, USA. angus.nairn@yale.edu.
Proteomes ; 6(4)2018 Dec 09.
Article en En | MEDLINE | ID: mdl-30544872
Cell-type-specific analysis has become a major focus for many investigators in the field of neuroscience, particularly because of the large number of different cell populations found in brain tissue that play roles in a variety of developmental and behavioral disorders. However, isolation of these specific cell types can be challenging due to their nonuniformity and complex projections to different brain regions. Moreover, many analytical techniques used for protein detection and quantitation remain insensitive to the low amounts of protein extracted from specific cell populations. Despite these challenges, methods to improve proteomic yield and increase resolution continue to develop at a rapid rate. In this review, we highlight the importance of cell-type-specific proteomics in neuroscience and the technical difficulties associated. Furthermore, current progress and technological advancements in cell-type-specific proteomics research are discussed with an emphasis in neuroscience.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Proteomes Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Proteomes Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza