Apoptosis induced by ursodeoxycholic acid in human melanoma cells through the mitochondrial pathway.
Oncol Rep
; 41(1): 213-223, 2019 Jan.
Article
en En
| MEDLINE
| ID: mdl-30542709
Ursodeoxycholic acid (UDCA) is a type of hydrophilic bile acid extracted from animal bile with a wide range of biological functions. The present results demonstrated that UDCA could effectively inhibit the proliferation of two human melanoma cell line (M14 and A375) with time and concentrationdependence. Following exposure to various concentrations of UDCA, M14 cells exhibited typical morphological changes and weaker ability of colony forming. Flow cytometry analysis demonstrated that UDCA could induce a decrease of mitochondrial membrane potential and an increase in reactive oxygen species (ROS) levels in M14 cells. The cell cycle was arrested in the G2/M phase, which was confirmed by the decrease of cyclindependent kinase 1 and cyclinB1 at the protein level. However, when M14 cells were treated with UDCA and ZVADFMK (caspase inhibitor) synchronously, the apoptosis rate of the cells was reduced significantly. In addition, it was demonstrated that UDCA induced apoptosis of human melanoma M14 cells through the ROStriggered mitochondrialassociated pathway, which was indicated by the increased expression of cleavedcaspase3, cleavedcaspase9, apoptotic protease activating factor1, cleavedpoly (ADPribose) polymerase 1 and the elevation of B cell lymphoma2 (Bcl2) associated X protein/Bcl2 ratio associated with apoptosis. Therefore, UDCA may be a potential drug for the treatment of human melanoma.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ácido Ursodesoxicólico
/
Apoptosis
/
Mitocondrias
Límite:
Humans
Idioma:
En
Revista:
Oncol Rep
Asunto de la revista:
NEOPLASIAS
Año:
2019
Tipo del documento:
Article
Pais de publicación:
Grecia