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Characterization of inflammatory infiltrate of ulcerative dermatitis in C57BL/6NCrl-Tg(HMGA1P6)1Pg mice.
De Biase, Davide; Esposito, Francesco; De Martino, Marco; Pirozzi, Claudio; Luciano, Antonio; Palma, Giuseppe; Raso, Giuseppina Mattace; Iovane, Valentina; Marzocco, Stefania; Fusco, Alfredo; Paciello, Orlando.
Afiliación
  • De Biase D; Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Italy.
  • Esposito F; CNR - Institute of Experimental Endocrinology and Oncology, c/o Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Italy.
  • De Martino M; CNR - Institute of Experimental Endocrinology and Oncology, c/o Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Italy.
  • Pirozzi C; Department of Pharmacy, University of Naples Federico II, Italy.
  • Luciano A; S.S.D. Sperimentazione Animale, Istituto Nazionale Tumori-IRCCS-"Fondazione G. Pascale", Naples, Italy.
  • Palma G; S.S.D. Sperimentazione Animale, Istituto Nazionale Tumori-IRCCS-"Fondazione G. Pascale", Naples, Italy.
  • Raso GM; Department of Pharmacy, University of Naples Federico II, Italy.
  • Iovane V; Department of Pharmacy, University of Salerno, Fisciano, Italy.
  • Marzocco S; Department of Pharmacy, University of Salerno, Fisciano, Italy.
  • Fusco A; CNR - Institute of Experimental Endocrinology and Oncology, c/o Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Italy.
  • Paciello O; Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Italy.
Lab Anim ; 53(5): 447-458, 2019 Oct.
Article en En | MEDLINE | ID: mdl-30522404
Ulcerative dermatitis (UD) is an idiopathic, spontaneous and progressive disease typically affecting C57BL/6 aged mice with an unknown aetiopathogenesis. For this study, we evaluated 25 cases of UD in C57BL/6NCrl-Tg(HMGA1P6)1Pg mice. Formalin-fixed, paraffin-embedded skin samples were submitted to morphological investigations. Immunohistochemical analysis was performed to characterize and quantify inflammatory cells using CD3, CD45/B220, CD4, CD8 and IL-17 antibodies. Mast cell-bound IgE was investigated by immunofluorescence, whereas serum and cryopreserved skin samples were collected for molecular analysis. Student's t-test (two-tailed) was performed to assess significant differences between the two groups. Affected skin showed extensive areas of ulceration and diffuse, severe and mixed inflammatory infiltrates. No relevant changes were observed in control mice. Immunohistochemical analysis showed a predominant CD3 + CD4 + leukocyte population with fewer CD45/B220 and IL-17 immunolabelled cells and mast cell-bound IgE. Increases in TNFα, IL-1ß and Il-6 mRNA expression were observed in the skin of affected animals compared to controls. Serum TNFα and IL-6 did not vary between affected and control mice. Inflammatory infiltrates and cytokine expression were consistent with both Th2/IgE and Th17 differentiation, a typical pattern of a type I hypersensitivity reaction. Overall, our data suggest an allergic-based aetiopathogenesis of UD in C57BL/6NCrl-Tg(HMGA1P6)1Pg mice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Úlcera Cutánea / Dermatitis Límite: Animals Idioma: En Revista: Lab Anim Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Úlcera Cutánea / Dermatitis Límite: Animals Idioma: En Revista: Lab Anim Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido