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Viruses control dominant bacteria colonizing the terrestrial deep biosphere after hydraulic fracturing.
Daly, Rebecca A; Roux, Simon; Borton, Mikayla A; Morgan, David M; Johnston, Michael D; Booker, Anne E; Hoyt, David W; Meulia, Tea; Wolfe, Richard A; Hanson, Andrea J; Mouser, Paula J; Moore, Joseph D; Wunch, Kenneth; Sullivan, Matthew B; Wrighton, Kelly C; Wilkins, Michael J.
Afiliación
  • Daly RA; Department of Microbiology, Ohio State University, Columbus, OH, USA.
  • Roux S; Joint Genome Institute, Walnut Creek, CA, USA.
  • Borton MA; Environmental Sciences Graduate Program, Ohio State University, Columbus, OH, USA.
  • Morgan DM; School of Earth Sciences, Ohio State University, Columbus, OH, USA.
  • Johnston MD; School of Earth Sciences, Ohio State University, Columbus, OH, USA.
  • Booker AE; Department of Microbiology, Ohio State University, Columbus, OH, USA.
  • Hoyt DW; Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA.
  • Meulia T; Molecular and Cellular Imaging Center, Ohio State University, Wooster, OH, USA.
  • Wolfe RA; Department of Microbiology, Ohio State University, Columbus, OH, USA.
  • Hanson AJ; Department of Civil, Environmental, and Geodetic Engineering, Ohio State University, Columbus, OH, USA.
  • Mouser PJ; Department of Civil and Environmental Engineering, University of New Hampshire, Durham, NH, USA.
  • Moore JD; Department of Civil, Environmental, and Geodetic Engineering, Ohio State University, Columbus, OH, USA.
  • Wunch K; Department of Civil and Environmental Engineering, University of New Hampshire, Durham, NH, USA.
  • Sullivan MB; Dow Microbial Control, Collegeville, PA, USA.
  • Wrighton KC; Dow Microbial Control, Houston, TX, USA.
  • Wilkins MJ; Department of Microbiology, Ohio State University, Columbus, OH, USA.
Nat Microbiol ; 4(2): 352-361, 2019 02.
Article en En | MEDLINE | ID: mdl-30510171
The deep terrestrial biosphere harbours a substantial fraction of Earth's biomass and remains understudied compared with other ecosystems. Deep biosphere life primarily consists of bacteria and archaea, yet knowledge of their co-occurring viruses is poor. Here, we temporally catalogued viral diversity from five deep terrestrial subsurface locations (hydraulically fractured wells), examined virus-host interaction dynamics and experimentally assessed metabolites from cell lysis to better understand viral roles in this ecosystem. We uncovered high viral diversity, rivalling that of peatland soil ecosystems, despite low host diversity. Many viral operational taxonomic units were predicted to infect Halanaerobium, the dominant microorganism in these ecosystems. Examination of clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) spacers elucidated lineage-specific virus-host dynamics suggesting active in situ viral predation of Halanaerobium. These dynamics indicate repeated viral encounters and changing viral host range across temporally and geographically distinct shale formations. Laboratory experiments showed that prophage-induced Halanaerobium lysis releases intracellular metabolites that can sustain key fermentative metabolisms, supporting the persistence of microorganisms in this ecosystem. Together, these findings suggest that diverse and active viral populations play critical roles in driving strain-level microbial community development and resource turnover within this deep terrestrial subsurface ecosystem.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Consorcios Microbianos / Yacimiento de Petróleo y Gas / Firmicutes Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacteriófagos / Consorcios Microbianos / Yacimiento de Petróleo y Gas / Firmicutes Tipo de estudio: Prognostic_studies Idioma: En Revista: Nat Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido