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Biosynthesis and characterization of copper oxide nanoparticles from indigenous fungi and its effect of photothermolysis on human lung carcinoma.
Saravanakumar, Kandasamy; Shanmugam, Sabarathinam; Varukattu, Nipun Babu; MubarakAli, Davoodbasha; Kathiresan, Kandasamy; Wang, Myeong-Hyeon.
Afiliación
  • Saravanakumar K; Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
  • Shanmugam S; Bioprocess and Biomaterials Laboratory, Department of Microbial Biotechnology, Bharathiar University, Coimbatore 641046, India.
  • Varukattu NB; Proteomics & Molecular Cell Physiology Lab, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
  • MubarakAli D; School of Life Sciences, B.S. Abdur Rahman Crescent Institute of Science and Technology, Chennai 600048, Tamil Nadu, India.
  • Kathiresan K; Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
  • Wang MH; Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea. Electronic address: mhwang@kangwon.ac.kr.
J Photochem Photobiol B ; 190: 103-109, 2019 Jan.
Article en En | MEDLINE | ID: mdl-30508758
In this report, copper oxide nanoparticles (TA-CuO NPs) were synthesized using cell-free extract of Trichoderma asperellum and assessed their photothermal induced anticancerous activity. The fungal mediated TA-CuO NPs was confirmed by the surface plasmon resonance at 285-295 nm. The amide (CO) and aromatic (CC) groups in secondary metabolites of the extract was found to be an encapsulating or reducing agents for TA-CuO NPs, as indicated by IR spectra. Crystalline nature by cubic face-centered structure of the TA-CuO NPs was confirmed by XRD and their size ranges from 10 to 190 nm and an average of 110 nm by particle size analyzer (PSA). The Ultra HRSEM study revealed spherical shaped TA-CuO NPs. The FETEM results were also in strong agreement with PSA and UHR SEM. The survey-scan spectrum of XPS indicated the presence of C1s (47.83%), Cu2p (16.11%), Na1s (2.2%) and O1s (33.86%). The cell death was significantly found higher in photothermal induced by near-infrared laser (TA-CuO NPs-NIR) treated than that of TA-CuO NPs treatment. The level of ROS (35.62%) was higher in the treated cells than that of the untreated control, in accordance with the nucleus damage and losses in the mitochondrial membrane potential (ΔΨm). The upregulation of Bcl-2 in the untreated cells and Cas-3 in TA-CuO NPs-NIR treated cells was confirmed by western blot analysis. This work agreed with the potential biogenic TA-CuO NPs for promising in vitro photothermolysis of cancer cells, for the development of anticancer nanotherapeutics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cobre / Nanopartículas del Metal / Terapia por Láser / Hongos / Neoplasias Pulmonares / Antineoplásicos Límite: Humans Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Suiza
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cobre / Nanopartículas del Metal / Terapia por Láser / Hongos / Neoplasias Pulmonares / Antineoplásicos Límite: Humans Idioma: En Revista: J Photochem Photobiol B Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Suiza