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Nicotinamide Promotes Cell Survival and Differentiation as Kinase Inhibitor in Human Pluripotent Stem Cells.
Meng, Ya; Ren, Zhili; Xu, Faxiang; Zhou, Xiaoxiao; Song, Chengcheng; Wang, Vivien Ya-Fan; Liu, Weiwei; Lu, Ligong; Thomson, James A; Chen, Guokai.
Afiliación
  • Meng Y; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Ren Z; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Xu F; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Zhou X; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Song C; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Wang VY; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, China; Cancer Centre, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Liu W; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China; Bioimaging and Stem Cell Core Facility, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
  • Lu L; Center of Interventional Radiology, Zhuhai Precision Medical Center, Zhuhai People's Hospital, Jinan University, Zhuhai, Guangdong 519000, China.
  • Thomson JA; Regenerative Biology, Morgridge Institute for Research, Madison, WI 53715, USA; Department of Cell & Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53706, USA.
  • Chen G; Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, China. Electronic address: guokaichen@umac.mo.
Stem Cell Reports ; 11(6): 1347-1356, 2018 12 11.
Article en En | MEDLINE | ID: mdl-30503259
Nicotinamide, the amide form of vitamin B3, is widely used in disease treatments and stem cell applications. However, nicotinamide's impact often cannot be attributed to its nutritional functions. In a vitamin screen, we find that nicotinamide promotes cell survival and differentiation in human pluripotent stem cells. Nicotinamide inhibits the phosphorylation of myosin light chain, suppresses actomyosin contraction, and leads to improved cell survival after individualization. Further analysis demonstrates that nicotinamide is an inhibitor of multiple kinases, including ROCK and casein kinase 1. We demonstrate that nicotinamide affects human embryonic stem cell pluripotency and differentiation as a selective kinase inhibitor. The findings in this report may help researchers design better strategies to develop nicotinamide-related stem cell applications and disease treatments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Niacinamida / Células Madre Pluripotentes / Inhibidores de Proteínas Quinasas Límite: Humans Idioma: En Revista: Stem Cell Reports Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Niacinamida / Células Madre Pluripotentes / Inhibidores de Proteínas Quinasas Límite: Humans Idioma: En Revista: Stem Cell Reports Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos