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Transcription factor FTZ-F1 and cis-acting elements mediate expression of CYP6BG1 conferring resistance to chlorantraniliprole in Plutella xylostella.
Li, Xiuxia; Shan, Chunyang; Li, Fen; Liang, Pei; Smagghe, Guy; Gao, Xiwu.
Afiliación
  • Li X; Department of Entomology, Anhui Agricultural University, Hefei, People's Republic of China.
  • Shan C; Department of Entomology, China Agricultural University, Beijing, People's Republic of China.
  • Li F; Department of Entomology, China Agricultural University, Beijing, People's Republic of China.
  • Liang P; Department of Entomology, China Agricultural University, Beijing, People's Republic of China.
  • Smagghe G; Department of Entomology, China Agricultural University, Beijing, People's Republic of China.
  • Gao X; Department of Plants and Crops, Ghent University, Ghent, Belgium.
Pest Manag Sci ; 75(4): 1172-1180, 2019 Apr.
Article en En | MEDLINE | ID: mdl-30471186
BACKGROUND: Cytochrome P450-mediated detoxification plays an important role in the development of insecticide resistance. Previous studies have demonstrated that overexpression of CYP6BG1 was responsible for permethrin resistance in Plutella xylostella, and our experiments also showed that upregulation of this gene is associated with chlorantraniliprole resistance in P. xylostella. However, the transcriptional regulation involved in the expression of CYP6BG1 remains unknown. To further investigate the regulation of CYP6BG1 expression, the promoters of this gene were cloned and analyzed from one susceptible and four different resistant populations of P. xylostella. RESULTS: First, the promoter region of P. xylostella CYP6BG1 was compared in five populations, and three types of 5'-flanking region were found. Second, the region between -562 and +49 of CYP6BG1 in a field population (TH) of P. xylostella showed the highest promoter activity and could be induced by chlorantraniliprole. Third, the transcriptional factor FTZ-F1, which is an orphan nuclear receptor and binds to the fushi tarazu (ftz) gene, was predicted by the online software Alggen and Jaspar. It was proved to regulate the expression of CYP6BG1 by RNAi. The expression levels of FTZ-F1 and CYP6BG1 could be induced by chlorantraniliprole and were significantly higher in the resistant populations. CONCLUSIONS: These data give a better understanding of the transcriptional regulation of an important insecticide detoxification enzyme gene, and therefore will help in understanding the molecular mechanisms of insecticide resistance in P. xylostella. © 2018 Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Resistencia a los Insecticidas / Proteínas de Insectos / Ortoaminobenzoatos / Familia 6 del Citocromo P450 / Insecticidas / Mariposas Nocturnas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Resistencia a los Insecticidas / Proteínas de Insectos / Ortoaminobenzoatos / Familia 6 del Citocromo P450 / Insecticidas / Mariposas Nocturnas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido