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Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation.
Pirone, Luciano; Pitzer, Joshua Edison; D'Abrosca, Gianluca; Fattorusso, Roberto; Malgieri, Gaetano; Pedone, Emilia Maria; Pedone, Paolo Vincenzo; Roop, Roy Martin; Baglivo, Ilaria.
Afiliación
  • Pirone L; Institute of Biostructures and Bioimaging, C.N.R, Naples, 80134, Italy.
  • Pitzer JE; Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
  • D'Abrosca G; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy.
  • Fattorusso R; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy.
  • Malgieri G; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy.
  • Pedone EM; Institute of Biostructures and Bioimaging, C.N.R, Naples, 80134, Italy.
  • Pedone PV; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy. paolov.pedone@unicampania.it.
  • Roop RM; Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA. ROOPR@ecu.edu.
  • Baglivo I; Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", Caserta, 81100, Italy. ilaria.baglivo@unicampania.it.
Sci Rep ; 8(1): 17238, 2018 11 22.
Article en En | MEDLINE | ID: mdl-30467359
MucR is a member of the Ros/MucR family of prokaryotic zinc-finger proteins found in the α-proteobacteria which regulate the expression of genes required for the successful pathogenic and symbiotic interactions of these bacteria with the eukaryotic hosts. The structure and function of their distinctive zinc-finger domain has been well-studied, but only recently the quaternary structure of the full length proteins was investigated demonstrating their ability to form higher-order oligomers. The aim of this study was to identify the region of MucR involved in higher-order oligomer formation by analysing deletion and point mutants of this protein by Light Scattering, and to determine the role that MucR oligomerization plays in the regulatory function of this protein. Here we demonstrate that a conserved hydrophobic region at the N-terminus of MucR is responsible for higher-order oligomer formation and that MucR oligomerization is essential for its regulatory function in Brucella. All these features of MucR are shared by the histone-like nucleoid structuring protein, (H-NS), leading us to propose that the prokaryotic zinc-finger proteins in the MucR/Ros family control gene expression employing a mechanism similar to that used by the H-NS proteins, rather than working as classical transcriptional regulators.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Brucella abortus / Regulación Bacteriana de la Expresión Génica Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Brucella abortus / Regulación Bacteriana de la Expresión Génica Idioma: En Revista: Sci Rep Año: 2018 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido