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Discovering proteins for chemoprevention and chemotherapy by curcumin in liver fluke infection-induced bile duct cancer.
Khoontawad, Jarinya; Intuyod, Kitti; Rucksaken, Rucksak; Hongsrichan, Nuttanan; Pairojkul, Chawalit; Pinlaor, Porntip; Boonmars, Thidarut; Wongkham, Chaisiri; Jones, Alun; Plieskatt, Jordan; Potriquet, Jeremy; Bethony, Jeffrey M; Mulvenna, Jason; Pinlaor, Somchai.
Afiliación
  • Khoontawad J; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Intuyod K; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Rucksaken R; Department of Thai Traditional Medicine, Faculty of Natural Resources, Rajamangala University of Technology Isan, Sakon Nakhon Campus, Sakon Nakhon, Thailand.
  • Hongsrichan N; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Pairojkul C; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Pinlaor P; Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, Thailand.
  • Boonmars T; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Wongkham C; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Jones A; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Plieskatt J; Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Potriquet J; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Bethony JM; Centre for Research and Development in Medical Diagnostic Laboratory, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
  • Mulvenna J; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
  • Pinlaor S; Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
PLoS One ; 13(11): e0207405, 2018.
Article en En | MEDLINE | ID: mdl-30440021
Modulation or prevention of protein changes during the cholangiocarcinoma (CCA) process induced by Opisthorchis viverrini (Ov) infection may become a key strategy for prevention and treatment of CCA. Monitoring of such changes could lead to discovery of protein targets for CCA treatment. Curcumin exerts anti-inflammatory and anti-CCA activities partly through its protein-modulatory ability. To support the potential use of curcumin and to discover novel target molecules for CCA treatment, we used a quantitative proteomic approach to investigate the effects of curcumin on protein changes in an Ov-induced CCA-harboring hamster model. Isobaric labelling and tandem mass spectrometry were used to compare the protein expression profiles of liver tissues from CCA hamsters with or without curcumin dietary supplementation. Among the dysregulated proteins, five were upregulated in liver tissues of CCA hamsters but markedly downregulated in the CCA hamsters supplemented with curcumin: S100A6, lumican, plastin-2, 14-3-3 zeta/delta and vimentin. Western blot and immunohistochemical analyses also showed similar expression patterns of these proteins in liver tissues of hamsters in the CCA and CCA + curcumin groups. Proteins such as clusterin and S100A10, involved in the NF-κB signaling pathway, an important signaling cascade involved in CCA genesis, were also upregulated in CCA hamsters and were then suppressed by curcumin treatment. Taken together, our results demonstrate the important changes in the proteome during the genesis of O. viverrini-induced CCA and provide an insight into the possible protein targets for prevention and treatment of this cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de los Conductos Biliares / Colangiocarcinoma / Curcumina / Proteómica Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de los Conductos Biliares / Colangiocarcinoma / Curcumina / Proteómica Límite: Animals / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Estados Unidos