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Phosphoinositide-specific phospholipase C in normal human liver and in alcohol abuse.
Fais, Paolo; Leopizzi, Martina; Di Maio, Valeria; Longo, Lucia; Della Rocca, Carlo; Tagliaro, Franco; Bortolotti, Federica; Lo Vasco, Vincenza Rita.
Afiliación
  • Fais P; Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, Bologna, Italy.
  • Leopizzi M; Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino-Sapienza University, Latina, Italy.
  • Di Maio V; Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino-Sapienza University, Latina, Italy.
  • Longo L; Department of Sensory Organs, Sapienza University of Rome, Rome, Italy.
  • Della Rocca C; Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino-Sapienza University, Latina, Italy.
  • Tagliaro F; Department of Medical and Surgical Sciences, Unit of Legal Medicine, University of Bologna, Bologna, Italy.
  • Bortolotti F; Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino-Sapienza University, Latina, Italy.
  • Lo Vasco VR; Department of Sensory Organs, Sapienza University of Rome, Rome, Italy.
J Cell Biochem ; 120(5): 7907-7917, 2019 May.
Article en En | MEDLINE | ID: mdl-30426534
The phosphoinositide (PI) signal transduction pathway participates in liver metabolism. Abnormal activity or expression of PI-specific phospholipase C (PLC) enzymes has been described in different liver diseases. We resume the role of the PI metabolism in liver and PLC abnormalities in different liver diseases. Moreover, we present the results of PLC analyses in a normal human liver and an alcohol-damaged liver. PLC enzymes and the expression of the corresponding genes in liver biopsies from individuals deceased for complications of the alcoholic liver disease (ALD) at different stages compared with normal controls (deceased individuals with histologically normal livers without alcohol addiction anamnesis) were analyzed by using immunohistochemistry and molecular biology techniques. The expression panel of PLCs was described in normal and alcohol abuse liver. Our observations suggest that the regulation of PLC expression might be due to posttranscriptional events and that alcohol affects the epigenetic control of PLC expression belonging to PI signaling. We also describe the alternate expression of PLCB1 and PLCH1 genes in liver. Our results corroborate literature data suggesting that PLC enzymes are differently expressed in normal versus pathological liver, playing a role in the histopathogenesis of liver tissue damage. The expression and/or localization of selected PLC isoforms is especially affected in alcohol-related liver tissue histopathology. Our present observations confirm that the modulation of protein synthesis plays a role in the regulation of PLC enzymes. We also suggest that this modulation might act at the transcription level. Further studies are required to investigate related epigenetic mechanisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Cell Biochem Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Cell Biochem Año: 2019 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos