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Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces.
Wang, Kai; Liu, Xiao-Fang; Bu, Qing-Ting; Zheng, Yang; Chen, Xin-Ai; Li, Yong-Quan; Mao, Xu-Ming.
Afiliación
  • Wang K; Institute of Pharmaceutical Biotechnology, Zhejiang University, Hangzhou, 310058, China.
  • Liu XF; Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolic Engineering, Hangzhou, 310058, China.
  • Bu QT; Institute of Pharmaceutical Biotechnology, Zhejiang University, Hangzhou, 310058, China.
  • Zheng Y; Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolic Engineering, Hangzhou, 310058, China.
  • Chen XA; Institute of Pharmaceutical Biotechnology, Zhejiang University, Hangzhou, 310058, China.
  • Li YQ; Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolic Engineering, Hangzhou, 310058, China.
  • Mao XM; Institute of Pharmaceutical Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Curr Microbiol ; 76(1): 95-99, 2019 Jan.
Article en En | MEDLINE | ID: mdl-30421143
Streptomyces are famed producers of secondary metabolites with diverse bioactivities and structures. However, biosynthesis of natural products will consume vast precursors from primary metabolism, and some secondary metabolites are toxic to the hosts. To overcome this circumstance and over-produce secondary metabolites, one of the strategies is to over-express biosynthetic genes under strong promoters specifically expressed during secondary metabolism. For this purpose, here based on Microarray and eGFP reporter assays, we obtained a promoter thlM4p, whose activity was undetectable in the first 2 days of fermentation, but sevenfold higher than the strong promoter ermE*p in the following days. Moreover, when the positive regulator gene scnRII was driven from thlM4p, natamycin yield increased 30% compared to ermE*p. Therefore, we provide a new way to identify promoters, which is silenced during primary metabolism while strongly expressed under secondary metabolism of Streptomyces.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Natamicina / Reactores Biológicos / Metabolismo Secundario Tipo de estudio: Diagnostic_studies Idioma: En Revista: Curr Microbiol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / Natamicina / Reactores Biológicos / Metabolismo Secundario Tipo de estudio: Diagnostic_studies Idioma: En Revista: Curr Microbiol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos