An automated solid-phase 17 alpha-hydroxyprogesterone ELISA method using a microtitre plate.

Yeo, K H; Elder, P A; Lewis, J G

Yeo, K H; Elder, P A; Lewis, J G.

Afiliación

Yeo KH; Department of Clinical Biochemistry, Christchurch Hospital, New Zealand.

Ann Clin Biochem ; 25 ( Pt 3): 293-7, 1988 May.

Article en En | MEDLINE | ID: mdl-3041905

RESUMEN

We report an automated ELISA method for the measurement of 17 alpha-hydroxyprogesterone in plasma samples. A rabbit antiserum raised against 17 alpha-hydroxyprogesterone-3-(O-carboxymethyl)oxime-bovine serum albumin is used for the assay and a homologous competitor 17 alpha-hydroxyprogesterone-3-(O-carboxymethyl)oxime-bovine thyroglobulin is coated onto a microtitre plate. A goat anti-rabbit IgG-horse radish peroxidase is used as a probe for this solid-phase assay. The assay exhibits good sensitivity, precision and accuracy. The method is used routinely for the management of patients with congenital adrenal hyperplasia.

Asunto(s)

Hidroxiprogesteronas/sangre; 17-alfa-Hidroxiprogesterona; Tampones (Química); Ensayo de Inmunoadsorción Enzimática; Humanos; Hidrólisis; Radioisótopos de Yodo; Péptido Hidrolasas; Radioinmunoensayo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidroxiprogesteronas Límite: Humans Idioma: En Revista: Ann Clin Biochem Año: 1988 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido

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