Your browser doesn't support javascript.
loading
Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases.
Silva, Mateus V C; Aguiar, Leandro G; de Castro, Heizir F; Freitas, Larissa.
Afiliación
  • Silva MVC; Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil.
  • Aguiar LG; Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil.
  • de Castro HF; Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil.
  • Freitas L; Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil. larissafreitas@usp.br.
World J Microbiol Biotechnol ; 34(11): 169, 2018 Nov 07.
Article en En | MEDLINE | ID: mdl-30406564
The parameters that effect the synthesis of poly(styrene-co-divinylbenzene) magnetized with magnetite (STY-DVB-M) by polymerization emulsion were assessed in order to obtain magnetic beads to be used as matrix for lipase immobilization. The combined effect of polyvinyl alcohol (PVA) concentration and agitation was studied using response surface methodology. A 22 full-factorial design was employed for experimental design and analysis of the results. The optimum PVA concentration and agitation were found to be 1 wt% and 400 rpm, respectively. These conditions allow attaining the best particle size distribution of the synthesized particles (80% between 80 and 24 mesh). The performance of the magnetic beads was tested as a matrix for immobilizing two microbial lipases (Lipases from Burkholderia cepacia-BCL and Pseudomonas fluorescens-AKL) by physical adsorption and high immobilization yields (> 70%) and hydrolytic activities (≅ 1850 U g-1) were attained. The properties of free and immobilized lipases were searched and compared. Similar performance regarding the analyzed parameters (biochemical properties, kinetic constants and thermal stability) were obtained. Moreover, both immobilized lipases were found to be able to catalyze the transesterification of coconut oil with ethanol to produce fatty acid ethyl esters (FAEE). Further study showed that the B. cepacia immobilized lipase could be used seven times without significant decrease of activity, revealing half-life time of 970 h.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Poliestirenos / Enzimas Inmovilizadas / Lipasa / Magnetismo Idioma: En Revista: World J Microbiol Biotechnol Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Poliestirenos / Enzimas Inmovilizadas / Lipasa / Magnetismo Idioma: En Revista: World J Microbiol Biotechnol Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania