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Longevity of Preserved Germplasm: The Temperature Dependency of Aging Reactions in Glassy Matrices of Dried Fern Spores.
Ballesteros, Daniel; Hill, Lisa M; Lynch, Ryan T; Pritchard, Hugh W; Walters, Christina.
Afiliación
  • Ballesteros D; USDA-ARS National Laboratory for Genetic Resources Preservation, 1111 South Mason Street, Fort Collins, CO, USA.
  • Hill LM; Center for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo and Botanical Garden, 3400 Vine Street, Cincinnati, OH, USA.
  • Lynch RT; Department of Comparative Plant and Fungal Biology, Royal Botanic Gardens, Kew, Wellcome Trust Millennium Building, Wakehurst Place, Ardingly, UK.
  • Pritchard HW; USDA-ARS National Laboratory for Genetic Resources Preservation, 1111 South Mason Street, Fort Collins, CO, USA.
  • Walters C; USDA-ARS National Laboratory for Genetic Resources Preservation, 1111 South Mason Street, Fort Collins, CO, USA.
Plant Cell Physiol ; 60(2): 376-392, 2019 Feb 01.
Article en En | MEDLINE | ID: mdl-30398653
This study explores the temperature dependency of the aging rate in dry cells over a broad temperature range encompassing the fluid to solid transition (Tg) and well below. Spores from diverse species of eight families of ferns were stored at temperatures ranging from +45�C to approximately -176�C (vapor phase above liquid nitrogen), and viability was monitored periodically for up to 4,300 d (∼12 years). Accompanying measurements using differential scanning calorimetry (DSC) provide insights into structural changes that occur, such as Tg between +45 and -20�C (depending on moisture), and triacylglycerol (TAG) crystallization between -5 and -35�C (depending on species). We detected aging even at cryogenic temperatures, which we consider analogous to unscheduled degradation of pharmaceuticals stored well below Tg caused by a shift in the nature of molecular motions that dominate chemical reactivity. We occasionally observed faster aging of spores stored at -18�C (conventional freezer) compared with 5�C (refrigerator), and linked this with mobility and crystallization within TAGs, which probably influences molecular motion of dried cytoplasm in a narrow temperature range. Temperature dependency of longevity was remarkably similar among diverse fern spores, despite widely disparate aging rates; this provides a powerful tool to predict deterioration of germplasm preserved in the solid state. Future work will increase our understanding of molecular organization and composition contributing to differences in longevity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esporas / Helechos Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Physiol Asunto de la revista: BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esporas / Helechos Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Physiol Asunto de la revista: BOTANICA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Japón