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PD-1 blockade inhibits lymphocyte apoptosis and restores proliferation and anti-viral immune functions of lymphocyte after CP and NCP BVDV infection in vitro.
Liu, Yu; Liu, Shanshan; He, Boning; Wang, Tian; Zhao, Shangqi; Wu, Chenhua; Yue, Shan; Zhang, Shixun; He, Mingrui; Wang, Li; Huang, Wenjing; Shi, Tongrui; Zhu, Zhanbo.
Afiliación
  • Liu Y; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China; Laboratory of Veterinary Microbiology, Veterinary Science Research Institute of HeiLongJiang Province, Qiqihar, 161006, China; Heilongjiang Provincial Engineering Technology Research
  • Liu S; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • He B; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Wang T; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Zhao S; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Wu C; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Yue S; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Zhang S; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • He M; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Wang L; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Huang W; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China.
  • Shi T; Laboratory of Veterinary Microbiology, Veterinary Science Research Institute of HeiLongJiang Province, Qiqihar, 161006, China.
  • Zhu Z; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing, 163319, China; Heilongjiang Provincial Engineering Technology Research Center for Prevention and Control of Cattle Diseases, Daqing, 163319, China. Electronic address: zhanbozhu@163.com.
Vet Microbiol ; 226: 74-80, 2018 Nov.
Article en En | MEDLINE | ID: mdl-30389046
Bovine viral diarrhea virus (BVDV) is an important virus that can cause extensive economic losses in both dairy and beef industry worldwide. Acute infection with BVDV results in peripheral blood lymphopenia, apoptosis and immunosuppression. Up-regulated programmed death-1 (PD-1) expression induces functional exhaustion of lymphocytes, inhibition of proliferation and apoptosis of lymphocytes during acute and chronic viral infections, such as HIV and HCV. However, there are no reports showing the role of PD-1 in peripheral blood lymphopenia, apoptosis and immunosuppression after acute BVDV infection. Accordingly, we measured the mRNA and protein expression of PD-1 and programmed death-ligand 1 (PD-L1) in peripheral blood mononuclear cells (PBMCs) infected with BVDV, and analyzed the effects of PD-1 blockade on immune-associated function and activity in peripheral blood lymphocytes (PBLs). The results showed that both cytopathic (CP) BVDV (strain NADL) and non-cytopathic (NCP) BVDV (strain KD) infection stimulated the mRNA and protein expression of PD-1 and PD-L1 significantly. The upregulation of PD-1/PD-L1 was accompanied by the decreased PBLs proliferation and increased apoptosis. Additionally, PD-1 blockade restored proliferation, inhibited apoptosis, increased IFN-γ production and decreased BVDV load. Remarkably, the PD-1/PD-L1 interaction has a more substantial effect on the immunoregulation of inhibiting proliferation induced by CP BVDV infection. Our findings confirm that PD-1 plays a vital role in peripheral blood lymphopenia and apoptosis caused by acute BVDV infection, and provide new insights into exploring the immunopathological mechanisms of BVDV or other members of the Flaviviridae family, and a potential therapeutic strategy to control BVDV infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Linfocitos / Virus de la Diarrea Viral Bovina Tipo 1 / Receptor de Muerte Celular Programada 1 Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Linfocitos / Virus de la Diarrea Viral Bovina Tipo 1 / Receptor de Muerte Celular Programada 1 Límite: Animals Idioma: En Revista: Vet Microbiol Año: 2018 Tipo del documento: Article Pais de publicación: Países Bajos