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Direct crystal formation from micronized bone and lactic acid: The writing on the wall for calcium-containing crystal pathogenesis in osteoarthritis?
Bulysheva, Anna A; Sori, Nardos; Francis, Michael P.
Afiliación
  • Bulysheva AA; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, United States of America.
  • Sori N; Old Dominion University, Department of Health Sciences, Norfolk, VA, United States of America.
  • Francis MP; Department of Biomedical Sciences, Eastern Virginia Medical School, Norfolk, VA, United States of America.
PLoS One ; 13(11): e0202373, 2018.
Article en En | MEDLINE | ID: mdl-30388106
INTRODUCTION: Pathological calcium-containing crystals accumulating in the joints, synovial fluid, and soft tissues are noted in most elderly patients, yet arthritic crystal formation remains idiopathic. Interestingly, elevated lactic acid and bone erosion are frequently among the comorbidities and clinical features of patients with highest incidence of crystal arthropathies. This work shows that bone particulates (modeling bone erosion) dissolve in lactic acid and directly generate crystals, possibly presenting a mechanism for crystal accumulation in osteoarthritis. METHODS AND RESULTS: Micronized human bone (average particle size of 160 µm x 79 µm) completely dissolved in lactic acid in 48 hours, and in synovial fluid with 500 mMol lactic acid in 5 days, generating birefringent rhomboid and rod-shaped crystals. SEM analysis with energy dispersive x-ray spectroscopy of these crystals showed average dimensions of around 2 µm x 40 µm, which contained oxygen, calcium and phosphorous at 8.64:1.85:1. Raman spectroscopy of the generated crystals further showed 910/cm and 1049/cm peaks, aligning with calcium oxalate monohydrate and calcium pyrophosphate, respectively. CONCLUSIONS: This work shows that lactic acid and micronized mineralized bone together directly generate calcium-containing crystals. These observations may provide insights into the elusive etiology of arthritis with crystal involvement, possibly indicating lactic acid as a clinical target for treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Huesos / Oxalato de Calcio / Pirofosfato de Calcio / Calcio / Ácido Láctico Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Aged / Aged80 / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoartritis / Huesos / Oxalato de Calcio / Pirofosfato de Calcio / Calcio / Ácido Láctico Tipo de estudio: Etiology_studies / Prognostic_studies Límite: Aged / Aged80 / Humans / Male Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos