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Screening and optimization of potential injection vehicles for storage of retinal pigment epithelial stem cell before transplantation.
Tian, Yangzi; Davis, Richard; Zonca, Michael R; Stern, Jeffrey H; Temple, Sally; Xie, Yubing.
Afiliación
  • Tian Y; Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York.
  • Davis R; Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York.
  • Zonca MR; Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York.
  • Stern JH; Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York.
  • Temple S; Department of Retina Research, Neural Stem Cell Institute, Rensselaer, New York.
  • Xie Y; Colleges of Nanoscale Science and Engineering, SUNY Polytechnic Institute, Albany, New York.
J Tissue Eng Regen Med ; 13(1): 76-86, 2019 01.
Article en En | MEDLINE | ID: mdl-30381899
Retinal pigment epithelial (RPE) cells are highly specialized neural cells that have several functions essential for vision. Progressive deterioration of RPE cells in elderly individuals can result in visual impairment and ultimately the blinding disease age-related macular degeneration. Subretinal transplantation of stem cell-derived RPE cell suspensions is being explored as a strategy to recover the damaged retina and improve vision. This approach may be improved by developing a vehicle that increases postinjection cell viability and distribution and integration of RPE cells. In this study, Food and Drug Administration-approved natural polymers, including alginate, methylcellulose, and hyaluronic acid (HA), were examined for performance as cell vehicles for adult human RPE stem cells (RPESCs). We compared the effect of RPESC storage as a cell suspension in these delivery vehicles for 1-96 hr at different temperatures on subsequent cell performance in a cell culture model. RPESC survival, attachment, distribution, proliferation, and differentiation into RPE cells were monitored by microscopy over the course of 8 weeks. Our in vitro results demonstrate that RPESC suspension in a 0.2% HA solution promotes better initial cell distribution, proliferation, cobblestone formation, and expression of RPE cell markers (microphthalmia-associated transcription factor and orthodenticle homeobox 2) after 96 hr of storage. These data suggest that HA addition to the vehicle can significantly enhance RPESC performance in vitro and is a promising strategy to pursue an improved delivery vehicle supporting in vivo RPE cell transplantation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación Biológica / Células Madre / Diferenciación Celular / Células Inmovilizadas / Técnicas de Cultivo de Célula / Epitelio Pigmentado de la Retina Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Aged / Humans Idioma: En Revista: J Tissue Eng Regen Med Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Preservación Biológica / Células Madre / Diferenciación Celular / Células Inmovilizadas / Técnicas de Cultivo de Célula / Epitelio Pigmentado de la Retina Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Aged / Humans Idioma: En Revista: J Tissue Eng Regen Med Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido