Your browser doesn't support javascript.
loading
Escherichia coli-Derived Outer Membrane Vesicles Deliver Galactose-1-Phosphate Uridyltransferase and Yield Partial Protection against Actinobacillus pleuropneumoniae in Mice.
Quan, Keji; Zhu, Zhuang; Cao, Sanjie; Zhang, Fei; Miao, Chang; Wen, Xintian; Huang, Xiaobo; Wen, Yiping; Wu, Rui; Yan, Qigui; Huang, Yong; Ma, Xiaoping; Han, Xinfeng; Zhao, Qin.
Afiliación
  • Quan K; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Zhu Z; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Cao S; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Zhang F; Sichuan Science-observation Experimental Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, P.R. China.
  • Miao C; National Teaching and Experimental Center of Animal, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Wen X; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Huang X; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Wen Y; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Wu R; Sichuan Science-observation Experimental Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, P.R. China.
  • Yan Q; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Huang Y; Sichuan Science-observation Experimental Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, P.R. China.
  • Ma X; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
  • Han X; Sichuan Science-observation Experimental Station of Veterinary Drugs and Veterinary Diagnostic Technology, Ministry of Agriculture, Chengdu, P.R. China.
  • Zhao Q; Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, P.R. China.
J Microbiol Biotechnol ; 28(12): 2095-2105, 2018 Dec 28.
Article en En | MEDLINE | ID: mdl-30380823
In our previous studies, we have identified several in vivo-induced antigens and evaluated their potential as subunit vaccine candidates in a murine model, in which the recombinant protein GalT showed the most potent immunogenicity and immunoprotective efficacy against Actinobacillus pleuropneumoniae. To exploit a more efficient way of delivering GalT proteins, in this study, we employed the widely studied E. coli outer membrane vesicles (OMVs) as a platform to deliver GalT protein and performed the vaccine trial using the recombinant GalT-OMVs in the murine model. Results revealed that GalT-OMVs could elicit a highly-specific, IgG antibody titer that was comparable with the adjuvant GalT group. Significantly higher lymphocyte proliferation and cytokines secretion levels were observed in the GalT-OMVs group. 87.5% and 50% of mice were protected from a lethal dose challenge using A. pleuropneumoniae in active or passive immunization, respectively. Histopathologic and immunohistochemical analyses showed remarkably reduced pathological changes and infiltration of neutrophils in the lungs of mice immunized with GalT-OMVs after the challenge. Taken together, these findings confirm that OMVs can be used as a platform to deliver GalT protein and enhance its immunogenicity to induce both humoral and cellular immune responses in mice.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: UTP-Hexosa-1-Fosfato Uridililtransferasa / Infecciones por Actinobacillus / Vacunas Bacterianas / Inmunización / Actinobacillus pleuropneumoniae / Transporte de Proteínas / Escherichia coli Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Microbiol Biotechnol Año: 2018 Tipo del documento: Article Pais de publicación: Corea del Sur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: UTP-Hexosa-1-Fosfato Uridililtransferasa / Infecciones por Actinobacillus / Vacunas Bacterianas / Inmunización / Actinobacillus pleuropneumoniae / Transporte de Proteínas / Escherichia coli Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Microbiol Biotechnol Año: 2018 Tipo del documento: Article Pais de publicación: Corea del Sur