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Rhazyaminine from Rhazya stricta Inhibits Metastasis and Induces Apoptosis by Downregulating Bcl-2 Gene in MCF7 Cell Line.
Iqbal, Waqas; Alkarim, Saleh; Kamal, Tahseen; Choudhry, Hani; Sabir, Jamal; Bora, Roop S; Saini, Kulvinder S.
Afiliación
  • Iqbal W; 1 Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Alkarim S; 1 Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Kamal T; 2 Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Choudhry H; 3 Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Sabir J; 1 Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Bora RS; 1 Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Saini KS; 1 Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Integr Cancer Ther ; 18: 1534735418809901, 2019.
Article en En | MEDLINE | ID: mdl-30373413
BACKGROUND: The role of alkaloids isolated from Rhazya stricta Decne (Apocynaceae family) (RS) in targeting genes involved in cancer and metastasis remains to be elucidated. OBJECTIVE: Identify and characterize new compounds from RS, which inhibit gene(s) involved in the survival, invasion, self-renewal, and metastatic processes of cancer cells. METHODS: Bioinformatics study was performed using HISAT2, stringtie, and ballgown pipeline to understand expressional differences between a normal epithelial cell line-MCF10A and MCF7. NMR and ATR-FTIR were performed to elucidate the structure of rhazyaminine (R.A), isolated from R stricta. Cell viability assay was performed using 0, 25, and 50 µg/mL of total extract of R stricta (TERS) and R.A, respectively, for 0, 24, and 48 hours, followed by scratch assay. In addition, total RNA was isolated for RNA- seq analysis of MCF7 cell line treated with R.A followed by qRT-PCR analysis of Bcl-2 gene. RESULTS: Deptor, which is upregulated in MCF7 compared with MCF10A as found in our bioinformatics study was downregulated by R.A. Furthermore, R.A effectively reduced cell viability to around 50% ( P < .05) and restricted cell migration in scratch assay. Thirteen genes, related to metastasis and cancer stem cells, were downregulated by R.A according to RNA- seq analysis. Additionally, qRT-PCR validated the downregulation of Bcl-2 gene in R.A-treated cells by less than 0.5 folds ( P < .05). CONCLUSION: R.A successfully downregulated key genes involved in apoptosis, cell survival, epithelial-mesenchymal transition, cancer stem cell proliferation, and Wnt signal transduction pathway making it an excellent "lead candidate" molecule for in vivo proof-of-concept studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Regulación hacia Abajo / Apoptosis / Genes bcl-2 / Apocynaceae Límite: Humans Idioma: En Revista: Integr Cancer Ther Asunto de la revista: NEOPLASIAS / TERAPEUTICA Año: 2019 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Regulación hacia Abajo / Apoptosis / Genes bcl-2 / Apocynaceae Límite: Humans Idioma: En Revista: Integr Cancer Ther Asunto de la revista: NEOPLASIAS / TERAPEUTICA Año: 2019 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos