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Detection of Fowlpox virus carrying distinct genome segments of Reticuloendotheliosis virus.
Joshi, Lok R; Bauermann, Fernando V; Hain, Kyle S; Kutish, Gerald F; Armién, Anibal G; Lehman, Chad P; Neiger, Regg; Afonso, Claudio L; Tripathy, Deoki N; Diel, Diego G.
Afiliación
  • Joshi LR; Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
  • Bauermann FV; Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
  • Hain KS; Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
  • Kutish GF; Department of Pathobiology, University of Connecticut, Storrs, CT, USA.
  • Armién AG; Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, MN, USA.
  • Lehman CP; South Dakota Department of Game, Fish, and Parks, Custer State Park, Custer, SD, USA.
  • Neiger R; Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA.
  • Afonso CL; Exotic and Emerging Avian Viral Diseases Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA, USA.
  • Tripathy DN; Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
  • Diel DG; Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, South Dakota, USA. Electronic address: diego.diel@sdstate.edu.
Virus Res ; 260: 53-59, 2019 01 15.
Article en En | MEDLINE | ID: mdl-30359622
Fowlpox virus (FWPV), the type species of the genus Avipoxvirus family Poxviridae, is a large double-stranded DNA virus that causes fowlpox in chickens and turkeys. Notably, sequences of the avian retrovirus reticuloendotheliosis virus (REV) are frequently found integrated into the genome of FWPV. While some FWPV strains carry remnants of the REV long terminal repeats (LTRs), other strains have been shown to contain insertions of nearly the full-length REV provirus in their genome. In the present study we detected heterogeneous FWPV populations carrying the REV LTR or the near full-length REV provirus genome in a Merriam's wild turkey (Meleagris gallopavo merriami). The bird presented papules distributed throughout the non-feathered areas of the head. Avipoxvirus-like virions were observed in the lesions by transmission electron microscopy and the presence of FWPV was confirmed by DNA sequencing. Metagenomic sequencing performed on nucleic acid extracted from the skin lesions revealed two FWPV genome populations carrying either a 197-nt remnant of the REV LTR or a 7939-nt long fragment corresponding to the full-length REV provirus. Notably, PCR amplification using primers targeting FWPV sequences flanking the REV insertion site, confirmed the natural occurrence of the heterogeneous FWPV genome populations in one additional clinical sample from another turkey affected by fowlpox. Additionally, sequencing of a historical FWPV isolate obtained from chickens in the US in 2000 also revealed the presence of the two FWPV-REV genome populations. Results here demonstrate distinct FWPV populations containing variable segments of REV genome integrated into their genome. These distinct genome populations are likely a result of homologous recombination events that take place during FWPV replication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pavos / Virus de la Reticuloendoteliosis / Viruela Aviar / Virus de la Viruela de las Aves de Corral Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pavos / Virus de la Reticuloendoteliosis / Viruela Aviar / Virus de la Viruela de las Aves de Corral Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Virus Res Asunto de la revista: VIROLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos