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Tetrahymena promotes interactive transfer of carbapenemase gene encoded in plasmid between fecal Escherichia coli and environmental Aeromonas caviae.
Matsushita, Mizue; Okubo, Torahiko; Hasegawa, Takaki; Matsuo, Junji; Watanabe, Takanori; Iwasaki, Sumio; Fukumoto, Tatsuya; Hayasaka, Kasumi; Akizawa, Kozi; Shimizu, Chikara; Yamaguchi, Hiroyuki.
Afiliación
  • Matsushita M; Faculty of Health Sciences, Department of Medical Laboratory Science, Hokkaido University Graduate School of Health Sciences, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
  • Okubo T; Faculty of Health Sciences, Department of Medical Laboratory Science, Hokkaido University Graduate School of Health Sciences, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
  • Hasegawa T; Faculty of Health Sciences, Department of Medical Laboratory Science, Hokkaido University Graduate School of Health Sciences, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
  • Matsuo J; Faculty of Health Sciences, Department of Medical Laboratory Science, Hokkaido University Graduate School of Health Sciences, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
  • Watanabe T; Faculty of Health Sciences, Department of Medical Laboratory Science, Hokkaido University Graduate School of Health Sciences, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
  • Iwasaki S; Hokkaido University Hospital, Nishi-5 Kita-14 Jo, Kita-ku, Sapporo, Hokkaido 060-8648, Japan.
  • Fukumoto T; Hokkaido University Hospital, Nishi-5 Kita-14 Jo, Kita-ku, Sapporo, Hokkaido 060-8648, Japan.
  • Hayasaka K; Hokkaido University Hospital, Nishi-5 Kita-14 Jo, Kita-ku, Sapporo, Hokkaido 060-8648, Japan.
  • Akizawa K; Hokkaido University Hospital, Nishi-5 Kita-14 Jo, Kita-ku, Sapporo, Hokkaido 060-8648, Japan.
  • Shimizu C; Hokkaido University Hospital, Nishi-5 Kita-14 Jo, Kita-ku, Sapporo, Hokkaido 060-8648, Japan.
  • Yamaguchi H; Faculty of Health Sciences, Department of Medical Laboratory Science, Hokkaido University Graduate School of Health Sciences, Nishi-5 Kita-12 Jo, Kita-ku, Sapporo, Hokkaido 060-0812, Japan.
Microbiol Immunol ; 62(11): 720-728, 2018 Nov.
Article en En | MEDLINE | ID: mdl-30357893
Tetrahymena can facilitate plasmid transfer among Escherichia coli or from E. coli to Salmonella Enteritidis via vesicle accumulation. In this study, whether ciliates promote the interactive transfer of plasmids encoding blaIMP-1 between fecal E. coli and environmental Aeromonas caviae was investigated. Both bacteria were mixed with or without ciliates and incubated overnight at 30°C. The frequency of plasmid-acquired bacteria was estimated by colony counts using an agar plate containing ceftazidim (CAZ) followed by determination of the minimum inhibitory concentration (MIC). Cultures containing ciliates interactively transferred the plasmid between E. coli and Aeromonas with a frequency of 10-4 to 10-5 . All plasmid-acquired bacteria showed a MIC against CAZ of >128 µg/mL and the plasmid transfer was confirmed by PCR amplification of the blaIMP-1 gene. Fluorescent observation showed that both bacteria accumulated in the same vesicle and that transwell sequestering significantly decreased the transfer frequency. Although ciliates preferentially ingested E. coli rather than A. caviae, both bacteria were co-localized into the same vesicles of ciliates, indicating that their meeting is associated with the gene transfer. Thus, ciliates interactively promote plasmid transfer between E. coli and A. caviae. The results of this study will facilitate control of the spread of multiple-antibiotic resistant bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Tetrahymena / Proteínas Bacterianas / Beta-Lactamasas / Transferencia de Gen Horizontal / Heces / Aeromonas caviae Idioma: En Revista: Microbiol Immunol Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plásmidos / Tetrahymena / Proteínas Bacterianas / Beta-Lactamasas / Transferencia de Gen Horizontal / Heces / Aeromonas caviae Idioma: En Revista: Microbiol Immunol Año: 2018 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia