Genome-wide analysis of genes encoding MBD domain-containing proteins from tomato suggest their role in fruit development and abiotic stress responses.

Parida, Adwaita Prasad; Raghuvanshi, Utkarsh; Pareek, Amit; Singh, Vijendra; Kumar, Rahul; Sharma, Arun Kumar

Parida, Adwaita Prasad; Raghuvanshi, Utkarsh; Pareek, Amit; Singh, Vijendra; Kumar, Rahul; Sharma, Arun Kumar.

Afiliación

Parida AP; Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India.
Raghuvanshi U; Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India.
Pareek A; Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India.
Singh V; Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India.
Kumar R; Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India.
Sharma AK; Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India. arun@genomeindia.org.

Mol Biol Rep ; 45(6): 2653-2669, 2018 Dec.

Article en En | MEDLINE | ID: mdl-30350236

RESUMEN

In tomato, DNA methylation has an inhibitory effect on fruit ripening. The inhibition of DNA methyltransferase by 5-azacytidine results in premature fruit ripening. Methyl CpG binding domain (MBD) proteins are the readers of DNA methylation marks and help in the recruitment of chromatin-modifying enzymes which affect gene expression. Therefore, we investigate their contribution during fruit development. In this study, we identified and analyzed 18 putative genes of Solanum lycopersicum and Solanum pimpinellifolium encoding MBD proteins. We also identified tomato MBD syntelogs in Capsicum annum and Solanum tuberosum. Sixty-three MBD genes identified from four different species of solanaceae were classified into three groups. An analysis of the conserved domains in these proteins identified additional domains along with MBD motif. The transcript profiling of tomato MBDs in wild-type and two non-ripening mutants, rin and Nr, indicated constructive information regarding their involvement during fruit development. When we performed a stage-specific expression analysis during fruit ripening, a gradual decrease in transcript accumulation in the wild-type fruit was detected. However, a very low expression was observed in the ripening mutants. Furthermore, many ethylene-responsive cis-elements were found in SlMBD gene promoters, and some of them were induced in the presence of exogenous ethylene. Further, we detected the possible role of these MBDs in abiotic stresses. We found that few genes were differentially expressed under various abiotic stress conditions. Our results provide an evidence of the involvement of the tomato MBDs in fruit ripening and abiotic stress responses, which would be helpful in further studies on these genes in tomato fruit ripening.

Asunto(s)

Dominio de Unión a CpG-Metil/genética; Solanum lycopersicum/genética; Capsicum/genética; Metilación de ADN/genética; Etilenos; Frutas/genética; Perfilación de la Expresión Génica; Regulación de la Expresión Génica de las Plantas/genética; Genes de Plantas/genética; Estudio de Asociación del Genoma Completo; Proteínas de Plantas/genética; Regiones Promotoras Genéticas/genética; Solanum tuberosum/genética; Estrés Fisiológico

Palabras clave

Abiotic stress; Epigenetics; Fruit ripening; Methyl CpG binding domain proteins; Solanaceae; Tomato

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Solanum lycopersicum / Dominio de Unión a CpG-Metil Idioma: En Revista: Mol Biol Rep Año: 2018 Tipo del documento: Article País de afiliación: India Pais de publicación: Países Bajos

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