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Identification of structural glycoprotein E2 domain critical to mediate replication of Classical Swine Fever Virus in SK6 cells.
Borca, M V; Holinka, L G; Ramirez-Medina, E; Risatti, G R; Vuono, E A; Berggren, K A; Gladue, D P.
Afiliación
  • Borca MV; ARS, USDA, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Electronic address: Manuel.Borca@usda.gov.
  • Holinka LG; ARS, USDA, Plum Island Animal Disease Center, Greenport, NY 11944, USA.
  • Ramirez-Medina E; Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA.
  • Risatti GR; Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA.
  • Vuono EA; ARS, USDA, Plum Island Animal Disease Center, Greenport, NY 11944, USA; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN, USA.
  • Berggren KA; ARS, USDA, Plum Island Animal Disease Center, Greenport, NY 11944, USA; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN, USA.
  • Gladue DP; ARS, USDA, Plum Island Animal Disease Center, Greenport, NY 11944, USA. Electronic address: Douglas.Gladue@usda.gov.
Virology ; 526: 38-44, 2019 01 02.
Article en En | MEDLINE | ID: mdl-30340154
Envelope glycoprotein E2 of Classical Swine Fever Virus (CSFV) is involved in several critical virus functions. To analyze the role of E2 in virus replication, a series of recombinant CSFVs harboring chimeric forms of E2 CSFV and Bovine viral diarrhea virus (BVDV) were created and tested for their ability to infect swine or bovine cell lines. Substitution of native CSFV E2 by BVDV E2 abrogates virus replication in both cell lines. Substitution of individual domains in CSFV Brescia E2 by the homologous from BVDV produces chimeras that efficiently replicate in SK6 cells with the exception of a chimera harboring BVDV E2 residues 93-168. Further mapping revealed a critical area in E2 required for CSFV replication in SK6 cells between protein residues 136-156. This is the first report categorically defining a discrete portion of E2 as essential to pestivirus infection in susceptible cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Proteínas del Envoltorio Viral / Infecciones por Pestivirus / Virus de la Diarrea Viral Bovina / Dominios Proteicos / Virus de la Fiebre Porcina Clásica Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Virology Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Replicación Viral / Proteínas del Envoltorio Viral / Infecciones por Pestivirus / Virus de la Diarrea Viral Bovina / Dominios Proteicos / Virus de la Fiebre Porcina Clásica Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Virology Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos