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Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking.
Anderson, Ruthellen H; Kerkvliet, Jason G; Otta, Jaelin J; Ross, Alan D; Leiferman, Patricia C; Hoppe, Adam D; Francis, Kevin R.
Afiliación
  • Anderson RH; Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, USA; Cellular Therapies and Stem Cell Biology Group, Sanford Research, Sioux Falls, SD, USA.
  • Kerkvliet JG; Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD, USA; BioSystems Networks and Translational Research Center, Brookings, SD, USA.
  • Otta JJ; Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, USA.
  • Ross AD; Sanford Medical Genetics Laboratory, Cytogenetics Division, Sanford Hospital, Sioux Falls, SD, USA.
  • Leiferman PC; Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, USA; Sanford Medical Genetics Laboratory, Cytogenetics Division, Sanford Hospital, Sioux Falls, SD, USA.
  • Hoppe AD; Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD, USA; BioSystems Networks and Translational Research Center, Brookings, SD, USA.
  • Francis KR; Sanford School of Medicine, University of South Dakota, Sioux Falls, SD, USA; Cellular Therapies and Stem Cell Biology Group, Sanford Research, Sioux Falls, SD, USA. Electronic address: kevin.francis@sanfordhealth.org.
Stem Cell Res ; 33: 95-99, 2018 12.
Article en En | MEDLINE | ID: mdl-30340091
The most highly studied endocytic pathway, clathrin-dependent endocytosis, mediates a wide range of fundamental processes including nutrient internalization, receptor recycling, and signal transduction. In order to model tissue specific and developmental aspects of this process, CRISPR/Cas9 genomic editing was utilized to fluorescently label the C-terminus of clathrin light chain A (CLTA) within the phenotypically normal, parental CRMi001-A human induced pluripotent stem cell line. Successfully edited cells were isolated by fluorescently activated cell sorting, remained karyotypically normal, and maintained their differentiation potential. This cell line facilitates imaging of endogenous clathrin trafficking within varied cell types.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clatrina / Células Madre Pluripotentes Inducidas / Sistemas CRISPR-Cas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Clatrina / Células Madre Pluripotentes Inducidas / Sistemas CRISPR-Cas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido