Surface Fluctuations and Coalescence of Nucleolar Droplets in the Human Cell Nucleus.

Caragine, Christina M; Haley, Shannon C; Zidovska, Alexandra

Caragine, Christina M; Haley, Shannon C; Zidovska, Alexandra.

Afiliación

Caragine CM; Center for Soft Matter Research, Department of Physics, New York University, New York, New York 10003, USA.
Haley SC; Center for Soft Matter Research, Department of Physics, New York University, New York, New York 10003, USA.
Zidovska A; Center for Soft Matter Research, Department of Physics, New York University, New York, New York 10003, USA.

Phys Rev Lett ; 121(14): 148101, 2018 Oct 05.

Article en En | MEDLINE | ID: mdl-30339413

RESUMEN

The nucleolus is a membraneless organelle embedded in chromatin solution inside the cell nucleus. By analyzing surface dynamics and fusion kinetics of human nucleoli in vivo, we find that the nucleolar surface exhibits subtle, but measurable, shape fluctuations and that the radius of the neck connecting two fusing nucleoli grows in time as r(t)â¼t^{1/2}. This is consistent with liquid droplets with low surface tension â¼10^{-6} N m^{-1} coalescing within an outside fluid of high viscosity â¼10^{3} Pa s. Our study presents a noninvasive approach of using natural probes and their dynamics to investigate material properties of the cell and its constituents.

Asunto(s)

Nucléolo Celular/fisiología; Núcleo Celular/fisiología; Modelos Biológicos; Nucléolo Celular/química; Núcleo Celular/química; Cromatina/química; Cromatina/fisiología; Células HeLa; Humanos; Reología

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nucléolo Celular / Núcleo Celular / Modelos Biológicos Límite: Humans Idioma: En Revista: Phys Rev Lett Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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