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Immunophenotypic Profile of CD34+ Subpopulations and Their Role in the Diagnosis and Prognosis of Patients with De-Novo, Particularly Low-Grade Myelodysplastic Syndromes.
Gardikas, Nikolaos; Vikentiou, Myrofora; Konsta, Evgenia; Kontos, Christos K; Papageorgiou, Sotirios G; Spathis, Aris; Bazani, Efthimia; Bouchla, Anthi; Kapsimali, Violetta; Psarra, Katherina; Foukas, Periklis; Dimitriadis, George; Pappa, Vasiliki.
Afiliación
  • Gardikas N; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Vikentiou M; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Konsta E; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Kontos CK; Department of Biochemistry and Molecular Biology, National and Kapodistrian University of Athens, Panepistimiopolis, Athens, Greece.
  • Papageorgiou SG; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Spathis A; 2nd Department of Pathology, School of Medicine, University of Athens, University General Hospital Attikon, Haidari, Greece.
  • Bazani E; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Bouchla A; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Kapsimali V; Department of Dermatology and Venereology, HIV/AIDS Unit, Andreas Syggros Hospital, Athens, Greece.
  • Psarra K; Department of Immunology and Histocompatibility, Evangelismos Hospital, Athens, Greece.
  • Foukas P; 2nd Department of Pathology, School of Medicine, University of Athens, University General Hospital Attikon, Haidari, Greece.
  • Dimitriadis G; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
  • Pappa V; Second Department of Internal Medicine and Research Institute, University General Hospital Attikon, Haidari, Greece.
Cytometry B Clin Cytom ; 96(1): 73-82, 2019 01.
Article en En | MEDLINE | ID: mdl-30334347
BACKGROUND: Myelodysplastic syndromes (MDS) are clonal hematopoietic stem cell disorders with unknown aetiology. Multiparameter flow cytometry (MFC) is being evaluated for the diagnosis and prognosis of MDS. METHODS: In the present study, five-color MFC was performed on bone marrow aspirates of 50 untreated patients, newly diagnosed with MDS and 27 age matched control samples. Patients were classified according to World Health Organization 2016, International Prognostic Scoring System (IPSS), and Revised IPSS (IPSS-R). RESULTS: Significantly higher CD133+/CD90-CD45weak, CD117+/TdT-CD45weak, and CD33+/MPO-neutrophil precursor percentages on CD34+ cells, as well as a significant decrease of lymphoid and erythroid precursors were observed in the group of MDS patients in comparison to controls. A new scoring system was based on these findings, which can be helpful in discriminating lower risk MDS patients, including those with normal karyotype (a subgroup of MDS with diagnostic challenges). In addition, an increased level of apoptosis of CD34+/CD117+ cells was identified as an independent favorable prognostic factor both for the risk of transformation to acute myeloid leukemia and for overall survival. CONCLUSIONS: A new scoring system based on the expression of immature cell antigens on CD34+ cells (by itself or in combination with the Ogata score) can discriminate lower risk MDS patients, including those with normal karyotype, from the normal control group. © 2018 International Clinical Cytometry Society.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndromes Mielodisplásicos / Inmunofenotipificación / Antígenos CD34 Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cytometry B Clin Cytom Año: 2019 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndromes Mielodisplásicos / Inmunofenotipificación / Antígenos CD34 Tipo de estudio: Diagnostic_studies / Etiology_studies / Prognostic_studies / Risk_factors_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Cytometry B Clin Cytom Año: 2019 Tipo del documento: Article País de afiliación: Grecia Pais de publicación: Estados Unidos