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Single-Cell Transcriptomics of Traced Epidermal and Hair Follicle Stem Cells Reveals Rapid Adaptations during Wound Healing.
Joost, Simon; Jacob, Tina; Sun, Xiaoyan; Annusver, Karl; La Manno, Gioele; Sur, Inderpreet; Kasper, Maria.
Afiliación
  • Joost S; Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, 14183 Huddinge, Sweden.
  • Jacob T; Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, 14183 Huddinge, Sweden.
  • Sun X; Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, 14183 Huddinge, Sweden.
  • Annusver K; Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, 14183 Huddinge, Sweden.
  • La Manno G; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.
  • Sur I; Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.
  • Kasper M; Department of Biosciences and Nutrition and Center for Innovative Medicine, Karolinska Institutet, 14183 Huddinge, Sweden. Electronic address: maria.kasper@ki.se.
Cell Rep ; 25(3): 585-597.e7, 2018 10 16.
Article en En | MEDLINE | ID: mdl-30332640
Epithelial tissues, such as the skin, rely on cellular plasticity of stem cells (SCs) from different niches to restore tissue function after injury. How these molecularly and functionally diverse SC populations respond to injury remains elusive. Here, we genetically labeled Lgr5- or Lgr6-expressing cells from the hair follicle bulge and interfollicular epidermis (IFE), respectively, and monitored their individual transcriptional adaptations during wound healing using single-cell transcriptomics. Both Lgr5 and Lgr6 progeny rapidly induced a genetic wound signature that, for Lgr5 progeny, included the remodeling of receptors to permit interactions with the wound environment, a property that Lgr6 progeny possessed even before wounding. When contributing to re-epithelialization, Lgr5 progeny gradually replaced their bulge identity with an IFE identity, and this process started already before Lgr5 progeny left the bulge. Altogether, this study reveals how different SCs respond and adapt to a new environment, potentially explaining cellular plasticity of many epithelial tissues.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Células Madre / Cicatrización de Heridas / Folículo Piloso / Epidermis / Análisis de la Célula Individual / Transcriptoma Límite: Animals / Female / Humans Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piel / Células Madre / Cicatrización de Heridas / Folículo Piloso / Epidermis / Análisis de la Célula Individual / Transcriptoma Límite: Animals / Female / Humans Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos