The small molecule AMBMP disrupts microtubule growth, ciliogenesis, cell polarity, and cell migration.
Cytoskeleton (Hoboken)
; 75(10): 450-457, 2018 10.
Article
en En
| MEDLINE
| ID: mdl-30315640
2-Amino-4-(3,4-[methylenedioxy]benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) is a small molecule that has been previously reported to be both a Wnt agonist and a microtubule (MT) regulator. Here we report a detailed analysis of AMBMPs effects on MTs and on MT associated cellular processes including cell polarity, ciliogenesis, and cell migration. Specifically, treatment of Xenopus embryos with AMBMP leads to defects similar to the MT depolymerizing drug nocodazole, including a failure to generate or polarize cilia (depending on the timing of treatment) and a loss of the cell movements associated with radial intercalation. The dramatic effect AMBMP has on basic MT based cellular functions suggests that its usefulness as a Wnt regulator is questionable. Moreover, it may be an important new tool for experimental or pharmacological manipulation of MTs.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pirimidinas
/
Movimiento Celular
/
Cilios
/
Benzodioxoles
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Microtúbulos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cytoskeleton (Hoboken)
Año:
2018
Tipo del documento:
Article
Pais de publicación:
Estados Unidos