Your browser doesn't support javascript.
loading
Label-free quantitative proteomic analysis of human periodontal ligament stem cells by high-resolution mass spectrometry.
Han, Na-Young; Hong, Ji-Youn; Park, Jong-Moon; Shin, Changsik; Lee, Saya; Lee, Hookeun; Yun, Jeong-Ho.
Afiliación
  • Han NY; Gachon Institute of Pharmaceutical Sciences, Gachon College of Pharmacy, Gachon University, Incheon, Korea.
  • Hong JY; Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Korea.
  • Park JM; Gachon Institute of Pharmaceutical Sciences, Gachon College of Pharmacy, Gachon University, Incheon, Korea.
  • Shin C; Department of Microbiology and Immunology, Penn State University College of Medicine and Milton Hershey Medical Center, Hershey, PA, USA.
  • Lee S; Department of Periodontology, College of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju, Korea.
  • Lee H; Gachon Institute of Pharmaceutical Sciences, Gachon College of Pharmacy, Gachon University, Incheon, Korea.
  • Yun JH; Department of Periodontology, College of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju, Korea.
J Periodontal Res ; 54(1): 53-62, 2019 Feb.
Article en En | MEDLINE | ID: mdl-30298515
BACKGROUND AND OBJECTIVES: Proteome analysis of periodontal ligament stem cells (PDLSCs) could be used to study the function of PDL tissue. We used a label-free quantitative proteomic technique to investigate differentially expressed proteins (DEPs) in human PDLSCs (hPDLSCs) compared to human bone marrow mesenchymal stem cells (hBMSCs) and identify proteins specific to hPDLSCs. MATERIAL AND METHODS: hPDLSCs (n = 3) and hBMSCs (n = 3) were cultured and harvested for protein extraction and trypsin digestion. The proteomes of both cell types were analyzed by nano-liquid chromatography/tandem mass spectrometry. DEPs in hPDLSCs compared to hBMSCs were detected by label-free quantification and evaluated through signal transduction pathway and gene ontology (GO) analysis. RESULTS: In total, 690 and 771 proteins were identified from hPDLSCs and hBMSCs, of which 561 proteins were in common and 124 DEPs were found between hPDLSCs and hBMSCs. Fifty-eight proteins were expressed at significantly higher levels in hPDLSCs, whereas 66 proteins were expressed at lower levels compared to hBMSCs. The more highly expressed proteins were associated with translation and initiating protein synthesis, and lower expressed proteins were related to cell aging and metabolic processes. Proteins unique to hPDLSCs and hBMSCs were associated with translation and metabolic processes, respectively. CONCLUSION: Our results demonstrate evidence of distinct differences in protein expression between hPDLSCs and hBMSCs by using label-free quantitative proteomic analysis which was the first attempt in this field. DEPs included previously reported hPDLSC marker proteins and novel marker candidates, such as microtubule-associated protein, CTP synthase 1 and stathmin, which could be the markers for developing periodontal disease diagnostics and therapies.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre / Proteínas Adaptadoras Transductoras de Señales / Espectrometría de Masas en Tándem Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Periodontal Res Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre / Proteínas Adaptadoras Transductoras de Señales / Espectrometría de Masas en Tándem Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Periodontal Res Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos