Isolation and characterization of a marine testosterone-degrading bacterium: Vibrio sp. N3.
Rev Argent Microbiol
; 51(2): 170-178, 2019.
Article
en En
| MEDLINE
| ID: mdl-30297081
Steroids, including testosterone, estrone, 17ß-estradiol, estriol and 17ß-ethinyl estradiol, are harmful not only to the population dynamics of aquatic life forms but also to public health. In this study, a marine testosterone-degrading bacterium (strain N3) was isolated from Nanao Island in the South China Sea. In addition, the strain could also use 17ß-estradiol (E2), 17ß-ethinyl estradiol (EE2), estriol (E3) or cholesterol as a sole carbon source. According to the 16S rRNA gene sequence analysis, strain N3 was identified as Vibrio sp. Further characterization showed that the strain is aerobic, gram-negative, and mobile and exhibits resistance to ampicillin, carbenicillin, penicillin and spectinomycin. For enhancing its capacity of testosterone degradation, the Plackett-Burman factorial design and the central composite design were used to optimize the culture condition. Under optimal conditions, 92% of testosterone was degraded by Vibrio sp. N3 in 48h.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testosterona
/
Vibrio
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Rev Argent Microbiol
Año:
2019
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Argentina