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Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium.
Gojgic-Cvijovic, G D; Jakovljevic, D M; Loncarevic, B D; Todorovic, N M; Pergal, M V; Ciric, J; Loos, K; Beskoski, V P; Vrvic, M M.
Afiliación
  • Gojgic-Cvijovic GD; Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, Belgrade, Serbia. Electronic address: ggojgic@chem.bg.ac.rs.
  • Jakovljevic DM; Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, Belgrade, Serbia.
  • Loncarevic BD; Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, Belgrade, Serbia.
  • Todorovic NM; Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, Belgrade, Serbia.
  • Pergal MV; Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, Belgrade, Serbia.
  • Ciric J; Charles River Laboratories, Den Bosch, the Netherlands.
  • Loos K; Zernike Institute for Advanced Materials, University of Groningen, Groningen, the Netherlands.
  • Beskoski VP; Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
  • Vrvic MM; Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Int J Biol Macromol ; 121: 142-151, 2019 Jan.
Article en En | MEDLINE | ID: mdl-30291934
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K2HPO4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g/L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum production of polysaccharide was achieved over a shorter time interval (48 h). The polysaccharide produced in molasses medium had a weight average molecular weight of 5.82 × 106 Da, degree of branching 12.68%, viscosity of 0.24 dL/g, and based on methylation analysis and NMR data, it did not significantly differ from levan obtained in the medium with 200 g/L sucrose.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melaza / Medios de Cultivo / Beta vulgaris / Bacillus licheniformis / Fructanos Idioma: En Revista: Int J Biol Macromol Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Melaza / Medios de Cultivo / Beta vulgaris / Bacillus licheniformis / Fructanos Idioma: En Revista: Int J Biol Macromol Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos