Your browser doesn't support javascript.
loading
A Glutathione Precursor Reduces Oxidative Injury to Cultured Embryonic Cardiomyocytes.
Peterson, Darryl R; Huang, Huiya; Peresada, Dmitriy; Sukowski, Ernest J; White, Carl; Stefanov, Gospodin; Schweig, Lorene; Vazzalwar, Ramesh.
Afiliación
  • Peterson DR; Disciplines of Physiology and Biophysics and.
  • Huang H; Medicine, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL; and.
  • Peresada D; Disciplines of Physiology and Biophysics and.
  • Sukowski EJ; Disciplines of Physiology and Biophysics and.
  • White C; Disciplines of Physiology and Biophysics and.
  • Stefanov G; Disciplines of Physiology and Biophysics and.
  • Schweig L; Department of Pediatrics, Division of Neonatology, Advocate Children's Hospital, Advocate Lutheran General Hospital, Park Ridge, IL.
  • Vazzalwar R; Department of Pediatrics, Division of Neonatology, Advocate Children's Hospital, Advocate Lutheran General Hospital, Park Ridge, IL.
Am J Ther ; 27(5): e431-e438, 2020.
Article en En | MEDLINE | ID: mdl-30277904
BACKGROUND: Newborn infants are highly vulnerable to oxidative stress. Following birth asphyxia, oxidative injury due to ischemia-reperfusion can result in significant brain and heart damage, leading to death or long-term disability. STUDY QUESTION: The study objective was to evaluate the effectiveness of antioxidant gamma-L-glutamyl-L-cysteine (γGlu-Cys) in inhibiting oxidative injury to cultured embryonic cardiomyocytes (H9c2 cells). STUDY DESIGN: Control and γGlu-Cys-treated (0.5 mM) H9c2 cells were incubated under 6-hour ischemic conditions followed by 2-hour simulated reperfusion. MEASURES AND OUTCOMES: To quantify oxidative stress-induced apoptosis sustained by cardiomyocytes, lactate dehydrogenase (LDH) release and the presence of cytosolic cytochrome c were measured, as well as the number of secondary lysosomes visualized under electron microscopy. RESULTS: Compared to controls, H9c2 cells coincubated with γGlu-Cys during ischemia-reperfusion exhibited a significant reduction in both LDH release into the incubation medium [23.88 ± 4.08 (SE) vs. 9.95 ± 1.86% of total; P = 0.02] and the number of secondary lysosomes [0.070 ± 0.009 (SD) vs. 0.043 ± 0.004 per µm; P = 0.01]. Inhibition of LDH release with γGlu-Cys was the same (P = 0.67) as that of a caspase inhibitor. The significant increase in cytosolic cytochrome c (P = 0.01) after ischemia-reperfusion simulation further supports γGlu-Cys's role in apoptosis prevention. CONCLUSIONS: It is concluded that the glutathione precursor γGlu-Cys protects cultured embryonic cardiomyocytes from apoptosis-associated oxidative injury.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Asfixia Neonatal / Daño por Reperfusión Miocárdica / Miocitos Cardíacos / Dipéptidos / Antioxidantes Tipo de estudio: Etiology_studies Límite: Animals / Humans / Newborn Idioma: En Revista: Am J Ther Asunto de la revista: TERAPEUTICA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Asfixia Neonatal / Daño por Reperfusión Miocárdica / Miocitos Cardíacos / Dipéptidos / Antioxidantes Tipo de estudio: Etiology_studies Límite: Animals / Humans / Newborn Idioma: En Revista: Am J Ther Asunto de la revista: TERAPEUTICA Año: 2020 Tipo del documento: Article Pais de publicación: Estados Unidos