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An In Situ Reversible Heterodimeric Nanoswitch Controlled by Metal-Ion-Ligand Coordination Regulates the Mechanosensing and Differentiation of Stem Cells.
Kang, Heemin; Zhang, Kunyu; Jung, Hee Joon; Yang, Boguang; Chen, Xiaoyu; Pan, Qi; Li, Rui; Xu, Xiayi; Li, Gang; Dravid, Vinayak P; Bian, Liming.
Afiliación
  • Kang H; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, China.
  • Zhang K; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, China.
  • Jung HJ; Department of Materials Science and Engineering, Northwestern University, Evanston, IL, 60208, USA.
  • Yang B; International Institute for Nanotechnology, Evanston, IL, 60208, USA.
  • Chen X; NUANCE Center, Northwestern University, Evanston, IL, 60208, USA.
  • Pan Q; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, China.
  • Li R; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, China.
  • Xu X; Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, China.
  • Li G; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, China.
  • Dravid VP; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, 999077, China.
  • Bian L; Department of Orthopaedics & Traumatology, Faculty of Medicine, The Chinese University of Hong Kong, Prince of Wales Hospital, Hong Kong, 999077, China.
Adv Mater ; 30(44): e1803591, 2018 Nov.
Article en En | MEDLINE | ID: mdl-30277606
In situ and cytocompatible nanoswitching by external stimuli is highly appealing for reversibly regulating cellular adhesion and functions in vivo. Here, a heterodimeric nanoswitch is designed to facilitate in situ switchable and combinatorial presentation of integrin-binding cell-adhesive moieties, such as Mg2+ and Arg-Gly-Asp (RGD) ligand in nanostructures. In situ reversible nanoswitching is controlled by convertible coordination between bioactive Mg2+ and bisphosphonate (BP) ligand. A BP-coated gold-nanoparticle monomer (BP-AuNP) on a substrate is prepared to allow in situ assembly of cell-adhesive Mg2+ -active Mg-BP nanoparticles (NPs) on a BP-AuNP surface via Mg2+ -BP coordination, yielding heterodimeric nanostructures (switching "ON"). Ethylenediaminetetraacetic acid (EDTA)-based Mg2+ chelation allows in situ disassembly of Mg2+ -BP NP, reverting to Mg2+ -free monomer (switching "OFF"). This in situ reversible nanoswitching on and off of cell-adhesive Mg2+ presentation allows reversible cell adhesion and release in vivo, respectively, and spatiotemporally controls cyclic cell adhesion. In situ heterodimeric assembly of dual RGD ligand- and Mg2+ -active RGD-BP-Mg2+ NP (switching "Dual ON") further tunes and promotes focal adhesion, spreading, and differentiation of stem cells. The modular nature of this in situ nanoswitch can accommodate various bioactive nanostructures via metal-ion-ligand coordination to regulate diverse cellular functions in vivo in reversible and compatible manner.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Nanotecnología / Mecanotransducción Celular / Células Madre Mesenquimatosas / Magnesio Límite: Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Nanotecnología / Mecanotransducción Celular / Células Madre Mesenquimatosas / Magnesio Límite: Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania