[The effect of the phage lambda ral gene on the level of synthesis of the EcoK restriction endonuclease beta-subunit]. / Vliianie gena ral faga lambda na uroven' sinteza beta-sub''edinitsy éndonukleazy restriktsii EcoK.
Mol Biol (Mosk)
; 20(6): 1638-44, 1986.
Article
en Ru
| MEDLINE
| ID: mdl-3027537
E. coli hsd genes were subcloned from lambda 642 (ral+) into lambda L47.1 vector (ral-after replacement). The influence of bacteriophage lambda ral gene on the expression efficiency of hsdS kappa, hsdM kappa genes was investigated. It was shown, that its presence in vitro enhanced the synthesis of beta-subunit, hsdM gene product, and the increase of modification in vivo was observed. It is proposed that the increase of modification rate of lambda phage fully unmodified DNA is connected with the appearance of E. coli DNA methylase consisting of beta- and gamma-subunits but lacking alpha-subunit.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Desoxirribonucleasas de Localización Especificada Tipo I
/
Enzimas de Restricción del ADN
/
Bacteriófago lambda
/
Genes Virales
/
Vectores Genéticos
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Rusia