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Effect of glutamine on heat-shock protein beta 1 (HSPB1) expression during myogenic differentiation in bovine embryonic fibroblast cells.
Kim, Young-Shin; Lee, Jae-Sung; Lee, Yoonseok; Kim, Won-Seob; Peng, Dong-Qiao; Bae, Mun-Hee; Jo, Yong-Ho; Baik, Myunggi; Lee, Hong-Gu.
Afiliación
  • Kim YS; 1Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul, 05029 Korea.
  • Lee JS; 2Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul, 05029 Korea.
  • Lee Y; 1Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul, 05029 Korea.
  • Kim WS; 2Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul, 05029 Korea.
  • Peng DQ; Department of Biotechnology, Hankyung National University, Anseong-si, Gyeonggi-do 17579 Korea.
  • Bae MH; 1Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul, 05029 Korea.
  • Jo YH; 2Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul, 05029 Korea.
  • Baik M; 1Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul, 05029 Korea.
  • Lee HG; 2Team of An Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul, 05029 Korea.
Food Sci Biotechnol ; 27(3): 829-835, 2018 Jun.
Article en En | MEDLINE | ID: mdl-30263808
The objective of this study was to examine the effects of glutamine on heat-shock protein beta 1 (HSPB1) expression in bovine embryonic fibroblast cells during myogenesis. First, to elucidate the role of glutamine on HSPB1 expression during myogenesis, we treated with glutamine in myogenic lineage determinant (MyoD) over-expressed bovine embryonic fibroblast cells (BEFS-MyoD cells). Second, knockdown of HSPB1 using small interference RNA was performed to evaluate whether muscle development by glutamine is dependent on HSPB1 in BEFS-MyoD cells. As a result, glutamine promoted the mRNA level of HSPB1, Myogenin, Desmin, and mTOR as well as myotube formation, and protein synthesis (p < 0.05). The inhibition of HSPB1 expression during myogenesis has shown to repress the expression of myogenic marker genes (MyoD, Myogenin, Desmin) (p < 0.01), formation of myotubes and protein synthesis (p < 0.05). According to the results, it is concluded that glutamine regulates HSPB1 expression during myogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Sci Biotechnol Año: 2018 Tipo del documento: Article Pais de publicación: Corea del Sur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Sci Biotechnol Año: 2018 Tipo del documento: Article Pais de publicación: Corea del Sur