Your browser doesn't support javascript.
loading
Neuronal nitric oxide synthase contributes to the normalization of blood pressure in medicated hypertensive patients.
Costa, Eduardo D; Silva, Josiane F; Aires, Rosária D; Garcia, Daniela C; Kansaon, Milhem J; Wainstein, Alberto J; Rezende, Bruno A; Teixeira, Mauro M; Silva, Rafaela F; Cortes, Steyner F; Lemos, Virginia S.
Afiliación
  • Costa ED; Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Silva JF; Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Aires RD; Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Garcia DC; Department of Pharmacology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Kansaon MJ; Department of Health Sciences, Post-graduate Institute, Medical Sciences College, Belo Horizonte, Brazil.
  • Wainstein AJ; Department of Health Sciences, Post-graduate Institute, Medical Sciences College, Belo Horizonte, Brazil.
  • Rezende BA; Department of Health Sciences, Post-graduate Institute, Medical Sciences College, Belo Horizonte, Brazil.
  • Teixeira MM; Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Silva RF; Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Cortes SF; Department of Pharmacology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Lemos VS; Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: vslemos@icb.ufmg.br.
Nitric Oxide ; 80: 98-107, 2018 11 01.
Article en En | MEDLINE | ID: mdl-30261273
Neuronal nitric oxide synthase (nNOS) is expressed in the cardiovascular system and besides NO, generates H2O2. nNOS has been proposed to contribute to the control of blood pressure in healthy humans. The aim of this study was to verify the hypothesis that nNOS can contribute to the control of vascular relaxation and blood pressure in hypertensive patients undergoing drug treatment. The study was conducted in resistance mesenteric arteries from 63 individuals, as follows: 1) normotensive patients; 2) controlled hypertensive patients (patients on antihypertensive treatment with blood pressure normalized); 3) uncontrolled hypertensive patients (patients on antihypertensive treatment that remained hypertensive). Only mesenteric arteries from uncontrolled hypertensive patients showed impaired endothelium-dependent vasorelaxation in response to acetylcholine (ACh). Selective nNOS blockade with inhibitor 1 and catalase, which decomposes H2O2, decreased vasorelaxation in the three groups. However, the inhibitory effect was greater in controlled hypertensive patients. Decreased eNOS expression was detected in both uncontrolled and controlled hypertensive groups. Interestingly nNOS expression and ACh-stimulated H2O2 production were greater in controlled hypertensive patients, than in the other groups. ACh-stimulated NO production was lower in controlled hypertensive when compared to normotensive patients, while uncontrolled hypertensive patients showed the lowest levels. Catalase and nNOS blockade inhibited ACh-induced H2O2 production. In conclusion, nNOS-derived H2O2 contributes to the endothelium-dependent vascular relaxation in human resistance mesenteric arteries. The endothelial dysfunction observed in uncontrolled hypertensive patients involves decreased eNOS expression and NO production. The normalization of vascular relaxation and blood pressure in controlled hypertensive patients involves increased nNOS-derived H2O2 and NO production.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido Nítrico Sintasa de Tipo I / Hipertensión / Arterias Mesentéricas Tipo de estudio: Observational_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Nitric Oxide Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Óxido Nítrico Sintasa de Tipo I / Hipertensión / Arterias Mesentéricas Tipo de estudio: Observational_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Nitric Oxide Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2018 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos