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Effects of an oral synbiotic on the gastrointestinal immune system and microbiota in patients with diarrhea-predominant irritable bowel syndrome.
Moser, Adrian Mathias; Spindelboeck, Walter; Halwachs, Bettina; Strohmaier, Heimo; Kump, Patrizia; Gorkiewicz, Gregor; Högenauer, Christoph.
Afiliación
  • Moser AM; Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
  • Spindelboeck W; Theodor Escherich Laboratory for Microbiome Research, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
  • Halwachs B; Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
  • Strohmaier H; Theodor Escherich Laboratory for Microbiome Research, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
  • Kump P; Theodor Escherich Laboratory for Microbiome Research, Medical University of Graz, Auenbruggerplatz 15, 8036, Graz, Austria.
  • Gorkiewicz G; Institute of Pathology, Medical University of Graz, Auenbruggerplatz 25, 8036, Graz, Austria.
  • Högenauer C; Center for Medical Research, Medical University of Graz, Stiftingtalstraße 24, 8010, Graz, Austria.
Eur J Nutr ; 58(7): 2767-2778, 2019 Oct.
Article en En | MEDLINE | ID: mdl-30251020
PURPOSE: Diarrhea-predominant irritable bowel syndrome (IBS-D) is a common functional gastrointestinal disorder. Probiotics and synbiotics have been shown to improve symptoms of IBS, although mechanisms of action are currently not understood. METHODS: We investigated the effects of a 4-week oral synbiotic treatment (OMNi-BiOTiC® Stress Repair) in ten IBS-D patients on gastrointestinal mucosal and fecal microbiota, mucosa-associated immune cells, and fecal short-chain fatty acids. The upper and lower gastrointestinal tracts were compared before and after a 4-week synbiotic treatment using endoscopic evaluation to collect mucosal specimens for FACS analysis and mucosal 16S rRNA gene analysis. In stool samples, analysis for fecal SCFAs using GC-MS, fecal zonulin using ELISA, and fecal 16S rRNA gene analysis was performed. RESULTS: Synbiotics led to an increased microbial diversity in gastric (p = 0.008) and duodenal (p = 0.025) mucosal specimens. FACS analysis of mucosal immune cells showed a treatment-induced reduction of CD4+ T cells (60 vs. 55%, p = 0.042) in the ascending colon. Short-chain fatty acids (acetate 101 vs. 202 µmol/g; p = 0.007) and butyrate (27 vs. 40 µmol/g; p = 0.037) were elevated in fecal samples after treatment. Furthermore, treatment was accompanied by a reduction of fecal zonulin concentration (67 vs. 36 ng/ml; p = 0.035) and disease severity measured by IBS-SSS (237 vs. 54; p = 0.002). CONCLUSIONS: Our findings indicate that a short-course oral synbiotic trial may influence the human gastrointestinal tract in IBS-D patients on different levels which are region specific.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tracto Gastrointestinal / Síndrome del Colon Irritable / Diarrea / Simbióticos / Microbiota / Microbioma Gastrointestinal Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Eur J Nutr Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2019 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tracto Gastrointestinal / Síndrome del Colon Irritable / Diarrea / Simbióticos / Microbiota / Microbioma Gastrointestinal Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: Eur J Nutr Asunto de la revista: CIENCIAS DA NUTRICAO Año: 2019 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Alemania