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An In Vitro Model for Assessing Corneal Keratocyte Spreading and Migration on Aligned Fibrillar Collagen.
Kivanany, Pouriska B; Grose, Kyle C; Yonet-Tanyeri, Nihan; Manohar, Sujal; Sunkara, Yukta; Lam, Kevin H; Schmidtke, David W; Varner, Victor D; Petroll, W Matthew.
Afiliación
  • Kivanany PB; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. pouriskakivanany@gmail.com.
  • Grose KC; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. kyle.grose94@gmail.com.
  • Yonet-Tanyeri N; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. yonetnihan@gmail.com.
  • Manohar S; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. sujal_manohar@hotmail.com.
  • Sunkara Y; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. yukta.sunkara@gmail.com.
  • Lam KH; Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. kevin.lam2@utdallas.edu.
  • Schmidtke DW; Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. david.schmidtke@utdallas.edu.
  • Varner VD; Department of Surgery, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. david.schmidtke@utdallas.edu.
  • Petroll WM; Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, USA. vdv@utdallas.edu.
J Funct Biomater ; 9(4)2018 Sep 21.
Article en En | MEDLINE | ID: mdl-30248890
BACKGROUND: Corneal stromal cells (keratocytes) are responsible for developing and maintaining normal corneal structure and transparency, and for repairing the tissue after injury. Corneal keratocytes reside between highly aligned collagen lamellae in vivo. In addition to growth factors and other soluble biochemical factors, feedback from the extracellular matrix (ECM) itself has been shown to modulate corneal keratocyte behavior. METHODS: In this study, we fabricate aligned collagen substrates using a microfluidics approach and assess their impact on corneal keratocyte morphology, cytoskeletal organization, and patterning after stimulation with platelet derived growth factor (PDGF) or transforming growth factor beta 1 (TGFß). We also use time-lapse imaging to visualize the dynamic interactions between cells and fibrillar collagen during wound repopulation following an in vitro freeze injury. RESULTS: Significant co-alignment between keratocytes and aligned collagen fibrils was detected, and the degree of cell/ECM co-alignment further increased in the presence of PDGF or TGFß. Freeze injury produced an area of cell death without disrupting the collagen. High magnification, time-lapse differential interference contrast (DIC) imaging allowed cell movement and subcellular interactions with the underlying collagen fibrils to be directly visualized. CONCLUSIONS: With continued development, this experimental model could be an important tool for accessing how the integration of multiple biophysical and biochemical signals regulate corneal keratocyte differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Funct Biomater Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: J Funct Biomater Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza