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Dimethylformamide is a novel nitrilase inducer in Rhodococcus rhodochrous.
Chhiba-Govindjee, V P; Mathiba, K; van der Westhuyzen, C W; Steenkamp, P; Rashamuse, J K; Stoychev, S; Bode, M L; Brady, D.
Afiliación
  • Chhiba-Govindjee VP; CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa.
  • Mathiba K; Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg, PO Wits 2050, South Africa.
  • van der Westhuyzen CW; CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa.
  • Steenkamp P; CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa.
  • Rashamuse JK; CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa.
  • Stoychev S; The Department of Science and Technology (DST), Meiring Naude Road, Brummeria, Pretoria, 0001, South Africa.
  • Bode ML; CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa.
  • Brady D; Molecular Sciences Institute, School of Chemistry, University of the Witwatersrand, Johannesburg, PO Wits 2050, South Africa.
Appl Microbiol Biotechnol ; 102(23): 10055-10065, 2018 Dec.
Article en En | MEDLINE | ID: mdl-30244278
Nitrilases are of commercial interest in the selective synthesis of carboxylic acids from nitriles. Nitrilase induction was achieved here in three bacterial strains through the incorporation of a previously unrecognised and inexpensive nitrilase inducer, dimethylformamide (DMF), during cultivation of two Rhodococcus rhodochrous strains (ATCC BAA-870 and PPPPB BD-1780), as well as a closely related organism (Pimelobacter simplex PPPPB BD-1781). Benzonitrile, a known nitrilase inducer, was ineffective in these strains. Biocatalytic product profiling, enzyme inhibition studies and protein sequencing were performed to distinguish the nitrilase activity from that of sequential nitrile hydratase-amidase activity. The expressed enzyme, a 40-kDa protein with high sequence similarity to nitrilase protein Uniprot Q-03217, hydrolyzed 3-cyanopyridine to produce nicotinic acid exclusively in strains BD-1780 and BD-1781. These strains were capable of synthesising both the vitamin nicotinic acid as well as ß-amino acids, a compound class of pharmaceutical interest. The induced nitrilase demonstrated high enantioselectivity (> 99%) in the hydrolysis of 3-amino-3-phenylpropanenitrile to the corresponding carboxylic acid.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodococcus / Dimetilformamida / Aminohidrolasas Idioma: En Revista: Appl Microbiol Biotechnol Año: 2018 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rhodococcus / Dimetilformamida / Aminohidrolasas Idioma: En Revista: Appl Microbiol Biotechnol Año: 2018 Tipo del documento: Article País de afiliación: Sudáfrica Pais de publicación: Alemania